RESEARCH CONCERNING THE INFLUENCE OF CAPACITATION CONDITIONS ON THE QUALITY OF SPERMATOZOA USED FOR IN VITRO FERTILIZATION

Abstract

The optimization of capacitation technique is based on the study of the influence factors of this process. Studying the influence of capacitation conditions on boar sperm quality we concentrated our research upon capacitation technique and method. We used a dilution and washing method (centrifugation), a migration method (swim-up) and a selective washing method (Percoll). The capacitation medium acts and interacts with the capacitation technique and in order to study these factors we used: Tyrode`s, TALP-HEPES and TCM 199 media. The results showed that the capacitation technique influences the parameters of the sperm used for embryo in vitro production and the technique depends on the quality of the raw semen.