Development of ACRS-PCR method for detection of single nucleotide polymorphism g.66493737C/T of the equine myostanin gene (MSTN)

Michal Gábor, Martina Miluchová, Anna Trakovická

Abstract


Myostatin, encoded by the MSTN gene, is a member of the TGF-β super family that regulates 33 skeletal muscle development in a range of mammalian species including the horse. The aim of this work was designed of
molecular-genetic method for detection of single nucleotide polymorphism  g.66493737C/T of the equine myostatine gene (MSTN) in the breeds of horses Slovak warmblood and Norik  and analysis of genotype. The SNP polymorphism C/T of the MSTN gene was studied in a group of 63 animals (50 Noriker horses and 13 Slovak warmblood horses). Genomic DNA was isolated from samples of hairs by commercial column kit QIAamp DNA Mini kit. The SNP g.66493737C/T was analyzed by ACRS-PCR method with restriction endonuclease RsaI. The allele C was detected  by two restriction fragments 139 bp and 28 bp and the allele T with one 167 bp fragment. In the analyzed population of  horses were detected the following frequency of alleles and genotypes for the SNP polymorphisms C/T of the MSTN gene. Frequencies of allele T and allele C were 0.8095 and 0.1905 and frequencies of genotypes were 0.0159 (genotype CC), 0.3492 (genotype CT) and 0.6349 (genotype TT).

Keywords


ACRS-PCR method, horse, MSTN gene, SNP g.66493737C/T

Full Text:

PDF

References


McPherron, A.C., and Lee, S.J., Double muscling in cattle due to mutations in the myostatin gene, Proceedings of the National Academy of Sciences USA 94, 1997, 12457–61.

Grobet, L., Martin, L.J., Poncelet, D., et al., A deletion in the bovine myostatin gene causes the double-muscled phenotype in cattle, Nature Genetics, 1997, 17, 71–4

Schuelke, M., Wagner, K.R., Stolz, L.E., Hubner, C., Riebel, T., Komen, W., Braun, T., Tobin, J.F., and Lee, S.J., Myostatin mutation associated with gross muscle hypertrophy in a child, New England Journal of Medicine, 2004, 350, 2682–8

Mosher, D.S., Quignon, P., Bustamante, C.D., Sutter, N.B., Mellersh, C.S., Parker, H.G., and Ostrander, E.A., A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs, PloS Genetics, 2007, 3, e79

Gu, J., Orr, N., Park, S.D., Katz, L.M., Sulimova, G., MacHugh, D.E., and Hill, E.W., A genome scan for positive selection in thoroughbred horses, PLoS ONE, 2009, 4, e5767

Kambadur, R., Sharma, M., Smith, T.P.L., and Bass, J.J., Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle, Genome Res., 1997, 7, 910–916

Gunn, H.M., Muscle, bone and fat productions and muscle distribution of thoroughbreds and quarter horses, In Equine exercise physiology 2: Proceedings of the Second International Conference on Equine Exercise Physiology; August 7-11 1986;San Diego, California, United States, Available at: http://www.iceep.org/pdf/iceep2/_1129101114_001.pdf.

Hill, E.W., Gu, J., Eivers, S.S., Fonseca, R.G., McGivney, B.A., Govindarajan, P., Orr, N., Katz, L.M., and MacHugh, D., A sequence polymorphism in MSTN predicts sprinting ability and racing stamina in thoroughbred horses, PLoS ONE, 2010, 5, e8645

Rozen, S., and Skaletsky, H., Primer3 on the WWW for general users and for biologist programmers, Methods Mol. Biol., 2000, 132, 365–386

Tozaki, T., Miyake, T., Kakoi, H., Gawahara, H., Sugita, S., Hasegawa, T., Ishida, N., Hirora, K., and Nakano, Y., A genome-wide association study for racing performances in Thoroughbreds clarifies a candidate region near the MSTN gene, Animal Genetics, 2010, 41(Suppl. 2), 28–35

Bower, M.A, McGivney, B,A, Campana, M.G, Gu, J., Andersson, L.S., Barrett, E., et al. Thegenetic origin and history of speed in the Thoroughbred racehorse, Nature Com-munications, 2012, 3, 643

Hill, E.W., McGivney, B.A., Gu, J., Whiston, R., and Machugh, D.E., A genome-wide SNP-association study confirms a sequence variant (g.66493737C > T) in the equine myostatin (MSTN) gene as the most powerful predictor of optimum racing distance for Thoroughbred racehorses, BMC Genomics, 2010, 11, 552


Refbacks

  • There are currently no refbacks.


Copyright (c)


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110