Antimicrobial activity of crude methanolic extracts from Ganoderma lucidum and Trametes versicolor

Lukáš Hleba

Abstract


In this paper the antimicrobial activity of crude methanolic extracts obtained from Ganoderma lucidum and Trametes versicolor were investigated. The antimicrobial activities of the extracts against E. coli, P. aeruginosa, S. epidermis, E. raffinosus, S. cerevisiae and C. albicans were determined by the microbroth dillution method according by EUCAST in 96-well microplates. Microorganisms were obtained from Czech Collection of Microorganisms. Absorbances after and before the experiment were subtracted, converted to binary system and obtained values to Probit analysis were used. Of the two macromycetes extracts tested, not all extracts showed antimicrobial activity in tested MICs range. The highest antimicrobial activity showed the both extracts to Saccharomyces cerevisiae. The less antimicrobial effects had the both macromycetes extracts to Staphylococcus epidermis. Antimicrobial activity of macromycetes methanolic crude extracts to others tested microorganisms showed no effect or used concentration could be higher.


Keywords


Antimicrobial activity, macromycetes methanolic extracts, MIC, Ganoderma lucidum, Trametes versicolor

Full Text:

PDF

References


Butler, M.S. 2004. The role of natural product chemistry in drug discovery. Journal of Natural Products, 67, 2141-2153.

Lam, K.S. 2007. New aspects of natural products in drug discovery. Trends in Microbiology, 15, 6279-6289.

Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teixeira, V., Martins, A., Pintado, M. 2012. A review on antimicrobial activity of mushrooms (Basidiomycetes) extracts and isolated compounds. Planta Med., 78, 1707-1718.

Sheena, N., Ajith, T. A., Mathew, A.T., Janardhanan, K.K. 2003. Antibacterial activity of three macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus occuring in South India. Pharm. Biol., 41, 564-567.

Quereshi, S., Pandey, A.K., Sandhu, S.S. 2010. Evaluation of antibaterial activity of different Ganoderma lucisum extracts. J. Sci. Res., 3, 9-13.

Gao, Y., Tang, W., Gao, H., Chan, E., Lan, J., Li, X., Zhou, S. 2005. Antimicrobial activity of medical mushrooms Ganoderma. Food Rev. Int., 21, 211-222.

Sullivan, R., Smith, J.E., Rowan, N.J. 2006. Medicinal mushrooms and cancer therapy: translating a traditional practise into Western medicine. Perspect. Biol. Med., 49, 159-170.

Pala, S.A. and Wani, A.H. 2011. Mushrooms: the entities with multifarious medicinal properties. J. Pharm. Res., 165, 496-500.

9. Horská, E. 2012. New Consumerism and Trends at the Food Market: within and Beyond Visegrad Borders. In Horská, E. Food Sciences & Business Studies. Nitra : SPU, 2012, 410 p. ISBN 978-80-552-0815-2.

Clinical and Laboratory Standard Institute. Performance standard for antimicrobial susceptibility testing; informational supplement M100-S21. 21th ed. Wayne, PA: CLSI; 2009.

Heleno, S.A., Ferreira, I.C.F.R., Esteves, A.P., Ćirić, A., Glamočlija, J., Martins, A., Soković, M., Queiroz, M.J.R.P. 2013. Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters. Food and Chemical Toxicology, 58, 95-100.

Suay, I., Arenal, F., Asensio, F.J., Basilio, A., Cabello, A.M., Díez, M.T., García, J.B., González del Val, A., Gorrochategui, J., Hermández, P., Peláez, F., Vincente, M.F. 2000. Screening of basidiomycetes for antimicrobial activities. Antoine van Leeuwenhoek Journal of Microbiology, 78(2), 129-140.

Venturini, M.E., Rivera, C.S., Gonzalez, C., Blanco, D. 2008. Antimicrobial activity of extracts of edible wild and cultivated mushrooms against foodborne bacterial strains. Journal of Food Protection, 8, 1701-1706.

Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teixeira, V., Martins, A., Pintado, M. 2012. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Medica, 78(16), 1707-1718.

Keypour, S., Riahi, H., Moradali, M. F., Rafati, H. 2008. Investigation of the Antibacterial Activity of a Chloroform Extract of Ling Zhi or Reishi Medicinal Mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae), from Iran. International Journal of Medicinal Mushrooms, 10(4), 345-349.

Keller, C., Maillard, M., Keller, J., Hostettmann, K. 2002. Screening of European fungi for antibacterial, antifungal, larvicidal, molluscicidal, antioxidant and free-radical scavenging activities and subsequent isolation of bioactive compounds. Pharmaceutical biology, 40(7), 518-525.

Al-Fatimi, M., Wurster, M., Kreisel, H., Lindequist, U. 2005. Antimicrobial, cytotoxic and antioxidant activity of selected basidiomycetes from Yemen. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 60(10), 776-780.

Balakumar, R., Sivaprakasam, E., Kavitha, D., Sridhar, S., Kumar, J. S. 2011. Antibacterial and antifungal activity of fruit bodies of Phellinus mushroom extract. International J. Biosc., 1(3), 72-77.

Nikolovska-Nedelkoska, D., Atanasova-Pančevska, N., Amedi, H., Veleska, D., Ivanova, E., Karadelev, M., Kungulovski, D. 2013. Screening of antibacterial and antifungal activities of selected Macedonian wild mushrooms. Zbornik Matice srpske za prirodne nauke, (124), 333-340.

Öztürk, M., Duru, M. E., Kivrak, Ş., Mercan-Doğan, N., Türkoglu, A., Özler, M. A. 2011. In vitro antioxidant, anticholinesterase and antimicrobial activity studies on three Agaricus species with fatty acid compositions and iron contents: A comparative study on the three most edible mushrooms. Food and Chemical Toxicology, 49(6), 1353-1360.

Reinoso, R., Cajas-Madriaga, D., Martínez, M., San Martín, A., Pérez, C., Fajardo, V., Becerra, J. 2013. Biological Activity Of Macromycetes Isolated From Chilean Subantarctic Ecosystems. Journal of the Chilean Chemical Society, 58(4), 2016-2019.

Kavitha, D., Balakumar, R., Sivaprakasam, E., Sridhar, S., Suresh, J. 2011. Antibacterial and antifungal potential of fruit body extracts from daldinia concentrica (bolton) cesati & de notaris. International journal of pharmaceutical sciences and research, 2(9), 2376-2379.

Zheng, H., Maoqing, Y., Liqiu, X., Wenjuan, T., Lian, L., Guolin, Z. 2006. Purification and characterization of an antibacterial protein from the cultured mycelia of Cordyceps sinensis. Wuhan University J. Nat. Sci., 11, 709-714.


Refbacks

  • There are currently no refbacks.


Copyright (c)


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110