Some Comparative Histomorphometrical Aspects Regarding Detoxifying Capacity of Garlic, Coriander and Chlorella, in Chronic Cd Contamination on Carassius gibelio Species

Mărioara Nicula, Gabi Dumitrescu, Monica Dragomirescu, Camelia Tulcan, Eliza Simiz, Liliana Petculesci-Ciochina, silvia erina, Adela Marcu


The purpose of this study was to histomorphometrically compare the aspect of gill, intestinal and ovarian epithelium of Prussian carp specimens, simultaneously subjected to chronic intoxication with Cd and, to chelating and antioxidant effect of chlorella, coriander and garlic on this toxicant respectively.

150 Prussian carps, 10-12 g of weight were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized coriander in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed).

Fragments of gill, small intestine and ovary were removed and analyzed by light microscopy and a specific QuickPHOTO Micro 2.2 software has been used for the histomorphometric study.

Mentioned epithelium suffered evident histomorphologic and histomorphometric changes under the action of Cd and Cd plus chlorella, Cd plus coriander and Cd plus garlic respectively. Statistical processing data related to the gill lamellae length, intestinal villi height and chorion thickness of ovaries follicles revealed the existence of different degrees of significance between experimental groups compared.


Cd intoxication, chlorella, coriander, freshwater fish, garlic, histomorphometric study

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Mason, C. F., Biology of freshwater pollution. 2nd ed. Longman, New York, 1991, pp. 351.

Linnik, P. M. and Zubenko, I. B., Role of bottom sediments in the secondary pollution of aquatic environments by heavy metal compounds, Lakes and Reservoirs Res. Manage., 2000, 5 (1), 11-21

Groundwork, 2002,

El-Morshedi, N., Alzahrani, I., Kizilbash, N. A., Abdeen, A., El-Shebbly, A.A., and El-Berri, A., Effect of Heavy Metal Pollutants on Fish Population in two Egyptian Lakes, International Journal of Advanced Research, 2014, 2 (1), 409

Ma, W., Wang, L., He, Y. and Yan, Y., Tissue-specific Cd and metallothionein levels in Sinopotamon henanense, Environmental Toxicology, 2007, 393-400

Giari, L., Manera, M., Simoni, E. and Dezfuli, B. S., Cellular alterations in different organs of Europeansea bass Dicentrarchus labrax (L.) exposed to cadmium, Chemosphere, 2007, 67 (6), 1171-1181

Krumschnabel, G., Ebner, H. L., Hess, M. W. and Villunger, A., Poptosis and necroptosis are induced in rainbow trout cell lines exposed to cadmium, Aquatic Toxicol., 2010, 99 (1), 73-85

Dangre, A. J, Manning, S. and Brouwer, Effects of cadmium on hypoxia-induced expression of hemoglobin and erythropoietin in larval sheepshead minnow, Cyprinodon variegates, Aquatic Toxicol., 2010, 99 (2), 168-175

Vesey, D. A., Transport pathways for cadmium in the intestine and kidney proximal tubule: Focus on interaction with essential metals, Toxicol. Letters, 2010, 198 (1), 13-19

International Agency for Research on Cancer (IARC) Agents Classified by the IARC Monographs., 2012,1-106


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