Enterobacteriaceae Genera Representation of Chicken’s Gastrointestinal Tract after Feed Additives Application

Martin Mellen, Miroslava Kačániová, Jana Petrová, Henrieta Arpášová, Marcela Capcarová, Peter Haščík


The aim of this study was to examine the effect of different feed additives on the Enterobacteriaceae genera colonization of chicken (Coob 500) gastrointestinal tract. The Agolin Poultry, AgolinTannin Plus, Biostrong 510+FortiBac and Agolin acid were administered to forth feed mixtures and Biocitro to drinking water in various amounts except of the control group. The addition of 1.5 g Agolin Poultry to 15 kg of feed was included in the first experimental group, the addition of 7.5 g Agolin Tannin Plus to 15 kg of feed in the second experimental group, the addition of 15g Biostrong510+FortiBac to 15 kg of feed the third experimental group, 15 g  in the fourth experimental group of Agolin acid to 15 kg of feed and 10 ml fifth experimental group of drinking water. The highest count of Enterobacteriaceae genera counts was found in the control group. The lower count of Enterobacteriaceae genera counts was found in the second experimental group where 7.5 g of Agolin Tannin Plus to 15 kg was added to feed mixture.


feed additives, gastrointestinal tract colonization, Enterobacteriaceae genera

Full Text:



Horská, E., Yespolov, T.I. et al., Sustainability in Business and Society: Global Challenges – Local Solutions. Krakow: Wydawnictwo Episteme, 2013, 166 p. ISBN 978-83-7759-015-7.

Fairbrother, J. M., Nadeau, E. & Gyles, C. L. Escherichia coli in post weaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies. Animal Health Research Revue, 2005, 6, pp. 17–39.

Kaper, J. B., Nataro, J. P. & Mobley, H. L. Pathogenic Escherichia coli. Natural Revue of Microbiology, 2004, 2, pp. 123–140.

Nagy, B., Fekete, P. Z. Enterotoxigenic Escherichia coli in veterinary medicine. International Journal of Medicinal Microbiology, 2005, 295, pp. 443–454.

Mountzouris, K.C., Tsirtsikos, P., Kalamara, E., Nitsch, S., Schatzmayr, G., Fegeros, K., Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poultry Science 2007, 86, 309–317.

Rehman, H., Vahjen, W., Awad, W.A., Zentek, J. Indigenous bacteria and bacterial metabolic products in the gastrointestinal tract of broilers. Archives of Animal Nutrition, 2007, 61, pp. 319–335.

Rehman, H., Rosenkranz, C., Böhm, J., Zentek, J., Dietary inulin affects the morphology but not the sodium dependent glucose and glutamine transport in the jejunum of broilers. Poultry Science, 2007b, 86, pp. 118–122.

Giannenas, I., Tontis, D., Tsalie, E., Chronis, E.F., Doukas, D., Kyriazakis, I. Influence of dietary mushroom Agaricus bisporus on intestinal morphology and microflora composition in broiler chickens. Research in Veterinary Science, 2010, 89, pp. 78–84.

Sirvydis, V., Bobinienė, R., Gudavičiūtė, Čepulienė, R., Semaška, V., Vencius, D., Kepalienė, I., Influence of a prebiotic feed additive on some biochemical indices of blood and intestinal microbiota of broiler chickens. Žemér Ūkio Mokslai, 2006, 4, pp. 57–62.

Hentges D. J., The protective function of the indigenous intestinal flora, Pediatric Infectious Disease, 1986, 5, pp. 17–20.

Verstegen, M. W. A., Diet Composition and Non-antibiotic Feeding Programs in Europe, 66 Minnesota Nutrition Conference. Minnesota, USA, 2005, pp. 20–21.

Lu, J., Idris, U., Harmon, B., Hofacre, Ch., Maurer, J.J., Lee, M.D., Diversity and Succession of the Intestinal Bacterial Community of the Maturing Broiler Chicken, Applied Environmental Microbiology, 2003, 69, (11), pp. 6816–6824.

Mead, G. C., Prospects for competitive exclusion treatment to control salmonellas and other foodborne pathogens in poultry, Veterinary Journal, 2000, 159, pp. 111–123.

Falk, P. G., Hooper, L. V., Midtvedt, T., Gordon. J.I. Creating and maintaining the gastrointestinal ecosystem: what we know and need to know from gnotobiology. Microbiology of Molecular Biology Revue, 2000, 62, pp. 1157–1170.

Knarreborg, A., M. A., Simon, R. M., Engberg, B. B., Jensen, Tannock, G. W., Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at

various ages. Applied Environmental Microbiology, 2002, 68, pp. 5918–5924.

van der Wielen, P. W., Keuzenkamp, D.A., Lipman, L.J., van Knapen, F., Biesterveld, S., Spatial and temporal variation of the intestinal bacterial community in commercially raised broiler chickens during growth. Microbial Ecology, 2002, 44, pp. 286–293.

Zhu, X. Y., Zhong, T. Pandya, Z., Joerger, R.D., 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Applied Environmental Microbiology, 2002, 68, pp. 124–137.


  • There are currently no refbacks.

Copyright (c)

Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110