Examination the expression pattern of HSP70 heat shock protein in chicken PGCs and developing genital ridge

Mahek Anand, Roland Tóth, Alayu Kidane, Alexandra Nagy, Bence Lázár, Eszter Patakiné Várkonyi, Krisztina Liptói, Elen Gocza

Abstract


Chicken Primordial Germ cells (PGCs) are emerging pioneers in the field of applied embryology and stem cell technology. Now-a-days transgenic chickens are promising models to study human disease pathophysiology and drug designing. However, most of the molecular mechanism, which govern the stemness and pluripotency of chicken PGCs, not known in details.

Recent studies have indicated the role of HSP70 in early embryonic development in many vertebrate species. Exposure of chicken to heat stress result in activation of heat shock factors which activate the transcription of HSP70. Exposure chicken eggs to acute heat stress effects HSP70 expression in PGCs and gonads. HSP70 helps in maintaining the integrity of chicken PGCs. A new emerging role of HSP70 in apoptosis has emerged.

In our lab, we aim to characterize the expression of cHsp70 in chicken PGCs and gonads during embryonic development by subjecting the parents to acute levels of heat stress. Chickens whose parents subjected to heat stress showed varied expression of cHsp70 and also improved thermo tolerance.

In the future we plan to study other factors and miRNAs, which is characterized as an emerging player in regulating heat shock protein response in chicken and also plays an important role in apoptosis.


Keywords


apoptosis, heat stress, HSP70, genital ridges, PGCs

Full Text:

PDF

References


Al-Zghoul, M. B., Dalab, A. E., Ababneh, M. M., Jawasreh, K. I., Al Busadah, K. A., Ismail, Z. B., Thermal manipulation during chicken embryogenesis results in enhanced Hsp70 gene expression and the acquisition of thermotolerance, Res Vet Sci., 2013, 95(2) 502-507

Xie, J., Tang, L., Lu L, Zhang, L., Xi, L., Liu, H., C., Odle, J., Luo, X., Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus), PLoS One, 2014, 9(7), e102204

Rupik, W., Jasik, K., Bembenek, J., Widłak, W., The expression patterns of heat shock genes and proteins and their role during vertebrate's development, Comp Biochem Physiol A Mol Integr Physiol, 2011, 159(4) 349-366

Hamburger, V., Hamilton, H. L., A series of normal stages in the development of the chick embryo, Journal of morphology, 1951, 88(1), 49–92


Refbacks

  • There are currently no refbacks.


Copyright (c)


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110