In vitro antimicrobial and antioxidant activity of two selected plants from Lamiaceae species

Miroslava Kačániová


The aim of the present study was to evaluate antimicrobial and antioxidant activity of Melissa officinalis L. and Mentha piperita L. from Lamiaceae species (The detection of antimicrobial activity was carried out by disc diffusion method and evaluation of minimal inhibition concentration against five Gram-negative bacteria: Escherichia coli CCM 3988, Klebsiella pneumoniae CCM 2318, Salmonella enterica subsp. enterica CCM 3807, Shigella sonei CCM 1373, Yersinia enterocolitica CCM 5671 and five Gram-positive bacteria: Bacillus thuringiensis CCM 19, Clostridium perfringens CCM 4435, Haemophilus influenzae CCM4456, Listeria monocytogenes CCM 4699, Staphylococcus aureus subsp. aureus CCM 2461. Antioxidant activity was determined by DPPH radical scavenging and phosphomolybdenum antioxidant methods. The content of polyphenols and flavonoids was measured by colorimetric methods. Both tested plants showed the highest antimicrobial activity against Gram-negative bacteria confirmed by both methods - disc diffusion and minimal inhibitory concentration. Additionally, both tested plants exhibited high antioxidant activity. The highest content of total polyphenol (37.37 mg GAE/g g) was identified in lemon balm, but flavonoids (33.90 µg QE/g) in peppermint.


lemon balm,peppermint thyme essential oil, antimicrobial activity, antioxidant activity, flavonoids, polyphenols

Full Text:



Müller, J., Convective drying of medicinal, aromatic and spice plants: a review, Stewart Postharvest Review, 2007, 3(4), 1-6

Mimica-Dukic, N., Bozin, B., Sokovic, M., Simin, N., Antimicrobial and antioxidant activities of Melissa officinalis L. (Lamiaceae) essential oil, Journal of Agricultural and Food Chemistry, 2004, 52(9), 2485-2489

Herodez, S.S., Hadolin, M., Skerget, M., Knez, Z., Solvent extraction study of antioxidants from Balm (Melissa officinalis L.) leaves, Food Chemistry, 2003, 80, 275-282

Chen, H., Zuo, Y., Deng, Y., Separation and determination of flavonoids and other phenolic compounds in cranberry juice by high performance liquid chromatography, Journal of Chromatography A, 2001, 913, 387-395

Singh, R., Shushni, M. A. M., Belkheir, A. Antibacterial and antioxidant activities of Mentha piperita L., Arabian Journal of Chemistry, 2015, 8, 322–328

İşcan, G., Kirimer, N., Kürkcüoğlu, M., Başer, H. C., Demirci, F., Antimicrobial screening of Mentha piperita essential oils. Journal of Agricultural and Food Chemistry, 2002, 50, 3943–3946

Rajinder, S., Muftah, A.M., Belkheir, S.A., Antibacterial and antioxidant activities of Mentha piperita L., Arabian Journal of Chemistry, 2015, 8, 322–328

Clinical and Laboratory Standard Institute. Performance standard for antimicrobial susceptibility testing. Wayne, PA: Clinical and Laboratory Standard Institute; 2009. [Online] Available from:

Sánchés – Moreno, C., Larrauri, A., Saura – Calixto, F., A procedure to measure the antioxidant efficiency of polyphenols, Journal of the Science and Food Agriculture, 1998, 76, 270-276

Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 1999, 269, 337-341

Singleton, V.L., Rossi, J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Ameican. Journal of Enology and Agriculture, 1965,144-158

Willett, W.C., Balancing life-style and genomics research for disease prevention, Science, 2002, 292, 695-698

Sartoratto, A., Machado, A.L.M., Delarmelina, C., Figueira, G.M., Duarte, M.C.T., Rehder, V.L.G., Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Brazilian Journal of Microbiology, 2004, 34(4), 1517-1520

Rasooli, I., Gachkar, L., Yadegarinia, D., Rezaei, M.B., Astaneh S.D.A., Antibacterial and antioxidant characterization of essential oils from Mentha piperita and Mentha spicata grown in Iran, Acta Alimentaria, 2008, 37(1), 41–52

Priya, A.M., Manikandan, M., Kalaiselvi, G., Arun, P., Chinnaswamy, P., Selvam,K., Screening of antibacterial activity of Mentha piperita L. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 2007, 9(4), 1049–1052

Abdellatif, F., Boudjella, H., Zitouni, A., Hassani, A., Chemical composition and antimicrobial activity of the essential oil from leaves of Algerian Melissa officinalis L., EXCLI Journal, 2014, 13, 772–781

Albayrak, S., Aksoy, A., Albayrak, S., Sagdic, O. In vitro antioxidant and antimicrobial activity of some Lamiaceae species, Iranian Journal of Science and Technology, 2013, A1, 1-9

Carnat, A.P., Carnat, A., Fraisse, D., Lamison, J.L. The aromatic and polyphenolic composition of lemon balm (Melissa officinalis L. subs. officinalis) tea. Pharmaceutica Acta Helvetiae, 1998, 72, 301-308

Brahmi, F., Hauchard, D., Guendouze, N., Madani, K., Kiendrebeogo, M., et al. Phenolic composition, in vitro antioxidant effects and tyrosinese inhitory activity ot three Algerian menthe species: Mentha spinaca L., Mentha pulegium L. and Mentha rotundifolia L. Huds (Lamiaceae). Indusrtiale Crops and Products, 2015, 74, 720-730

Sadraei, H., Ghannadi, A., Malekshahi, K., Relaxant effect of essential oil of Melissa officinalis and citral on rat ileum contractions, Fitoterapia, 2003, 74, 445-452

McLafferty, F., Stauffer, D., The Important Peak Index of the Registry of Mass Spectral Data, Wiley, New York. 1991, 1

Yadegarinia, D., Gachkar, L., Bagher-Rezaei, M., Taghizadeh, M., Alipoor-Astaneh, S., Rasooli I., Biochemical activities of Iranian Mentha piperita L. and Myrtus communis L. essential oils, Phytochemistry 2006, 67,1249-1255

Samarth, R., Kumar, A., Radioprotection of Swiss albino mice by plant extract Mentha piperita (Linn.), Journal of Radiation Research, 2003, 44, 101-109

Mimica-Dukic, N., Bozin, B., Sokovic, M., Mihajlovic, B., Matavulj, M., Antimicrobial and antioxidant activities of three Mentha species essential oils, Planta Medica, 2003, 69, 413-419

Dorman, H.J.D., Kosar, M., Kahlos, K., Holm, Y., Hiltunen, R., Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars, Journal of Agriculture and Food Chemistry, 2003, 51, 4563-4569


  • There are currently no refbacks.

Copyright (c) 2016 Scientific Papers Animal Science and Biotechnologies

Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at]
Phone: +40-256-277160, Fax.: +40-256-277110