Effect of Thymus serpyllum and Ocimum basilicum essential oils on the shelf-life of chicken’s meat during refrigerated storage
Abstract
The aim of this study was to investigate the microbiological quality of chicken’s thighs meat after application of Thymus serpyllum and Ocimum basilicum essential oils with combination of vacuum packaging and EDTA treatment. The microbiological quality of control samples without vacuum packaging, vacuum packed with and without EDTA treatment, vacuum packed and treated with EDTA and samples treated with the essential oils of basil and breckland thyme were followed for 16 days. Microbiological analyses were conducted with standard microbiological methods. For anaerobic plate count, PCA agar was inoculated and incubated for 2 days at 35°C anaerobically. Pseudomonas spp. count was determined on Pseudomonas Isolation agar after incubation at 48 h at 25°C. For lactic acid bacteria (LAB), MRS agar was inoculated and incubated for 48-78 h at 37°C microaerophilly. For Enterobacteriaceae, VRBG agar was inoculated and incubated at 37°C for 24 h. . Anaerobic plate count ranged from 2.89 log CFU/g in all tested group on 0 day to 5.19 log CFU/g on 16 day in control group stored in air. LAB count ranged from 3.01 log CFU/g in all tested group on 0 day to 4.08 log CFU/g on 16 day in control group stored in air. Enterobacteriacea counts were from 1.33 log CFU/g on 0 day to 5.11 on 16 day in control group stored in air. Pseudomonas spp. were found only in control group stored in air on 12 and 16 day. The results of the present study suggest the possibility of application of Thymus serpyllum and Ocimum basilicum essential oils as natural food preservatives and potential sources of antimicrobial ingredients for food industry.
Keywords
Full Text:
PDFReferences
Patsias, A., Badeka, A.V., Savvaidis, I.N., Kontominas, M.G., Combined effect of freeze chilling and MAP on quality parameters of raw chicken fillets, Food Microbiology, 2008, 25, 575-581
Petrou, S., Tsiraki, M., Giatrakou, V., Savvaidis, I.N., Chitosan dipping or oregano oil treatments, singly or combined on modified atmosphere packaged chicken breast meat, International Journal of Food Microbiology, 2012, 156, 264-271
Mead, G.C., Microbiological quality of poultry meat: a review, Brazilian Journal of Poultry Science, 2004, 6 (3), 135-142.
Stahl-Biskup, E., Saez, F., Thyme, the Genus Thymus, Taylor and Francis, London, 2002, pp. 331
Ghasemi, A.P., Medicinal plants used in Chaharmahal and Bakhtyari districts, Iranian Herba Polonica, 2009, 55, 69-75
Nickavar, B., Mojab, F., Dolatbadi, R., Analysis of the essential oils of two Thymus species from Iran, Food Chemistry, 2005, 90, 609-611
Pirbalouti, A.G., Hashemi, M., Ghahfarokhic, F.T., Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops Products, 2013, 48, 43-48
Nikolic, M., Glamoclij, J., Ferreira, I.C.F.R., Calhelha, C.R., Fernandes, A., Markovic, T., Markovic, D., Giweli, A., Sokovic, M., Chemical composition, antimicrobial, antioxidant and antitumor activity of Thymus serpyllum L., Thymus algeriensis Boiss. and Reut and Thymus vulgaris L. essential oils. Industrial Crops and Products, 2014, 52, 183–190
Anamika, P. K., Muralidharan, P., Prakash, R., Thenmozhi, M., Teja, S., Aswini, M., Evaluation of ethanolic extract of Ocimum tenuiflorum for antiepileptic activity. Indian Journal of Pharmacology, 2013, 45, S235
Kaya, I., Yigit, N., Benli, M., Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy. African Journal of Traditional, Complementary and Alternative Medicines, 2008, 5(4), 363-369
Mahajan, N., Rawal, S., Verma, M., Poddar, M., Alok, S., A phytopharmacological overview on Ocimum species with special emphasis on Ocimum sanctum, Biomedicine & Preventive Nutrition, 2013, 3(2), 185-192
Snyder, O.P., Antimicrobial effects of spices and herbs. Hospitality Institute of Technology and Management, St. Paul, Minnaesota. http ://www.hitm.com/Documents/Spices.html. 1997
Shelef, L.A., Antimicrobial effects of spices. Journal of Food Safety, 1983, 6, 29-44
Hirasa, K., Takemasa, M., Spices Science and Technology. Marcel Dekker Inc. New York, USA, 1998, pp.1-232
Nevas, M., Korhonen, A.R., Lindtrom, M., Turkki, P., Korkeala, H., Antibacterial efficiency of Finnish spices essential oils against pathogenic and spoilage bacteria, Journal of Food Protection, 2004, 67, 199-202
Kim, J., Marshall, M. R., Wei, C., Antibacterial activity of some essential oil components against five food borne pathogens, Journal of Agriculture and Food Chemistry, 1994, 43, 2839-2845
Paster, N., Menasherov, M., Ravid, U., Juven, B., Antifungal activity of oregano and thyme essential oils applied as fumigants against fungi attacking stored grain, Journal of Food Protection, 1995, 58, 81-85
Petrová, J., Terentjeva, M., Puchalski, C., Hutková, J., Kántor, A., Mellen, M., Čuboň, J., Haščík, P., Kluz, M., Kordiaka, R., Kunová, S., Kačániová, M., Application of lavender and rosemary essential oils improvement of the microbiological quality of chicken quarters, Potravinárstvo, 2015, 9(1), 530-537
Kluz, M., Terentjeva, M., Puchalski, C., Hutková, J., Kántor, A., Petrová, J., Mellen, M., Čuboň, J., Haščík, P., Kordiaka, R., Kunová, S., Kačániová, M., The extension of shelf-life of chicken meat after application of caraway and anise essential oils and vacuum packaging, Potravinárstvo, 20l6, 10(1), 132-138
Kačániová, M., Terentjeva, M., Puchalski, C., Petrová, J., Hutková, J., Kántor, A., Mellen, M., Čuboň, J., Haščík, P., Kluz, M., Kordiaka, R., Kunová, S., Microbiological quality of chicken thighs meat after four essential oils combination, EDTA, vacuum packing, Potravinárstvo, 2016, 10(1), 107-113
Chouliara, E., Karatapanis, A., Savvaidis, I. N., Kontominas, M. G., Combined effect of oregano essential oil, modified atmosphere packaging on shelf-life extension of fresh chicken breast meat, stored at 4 °C, Food Microbiology, 2007, 24, 607-617
Refbacks
- There are currently no refbacks.
Copyright (c)
Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).
LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)
ISSN online 2344 - 4576
ISSN-L 1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)
PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________
Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania
E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110