The Use of Sea Buckthorn (Hippophae rhamnoides L.) and Milk Thistle (Silybum marianum L.) in Alloxan Induced Diabetes Mellitus in Rats

Florin Muselin, Diana Brezovan, Jelena Savici, Romeo T. Cristina, Eugenia Dumitrescu, Alexandru Octavian Doma, Doru Morar, Alexandra Trif


The study emphasize the effect of H. rhamnoides and S. marianum 6% aqueous extract on blood sugar level in Alloxan induced diabetes mellitus in rats. The rats (200g and 3 months age) were divided in five groups: one non-diabetic control and four experimental groups with induced diabetes mellitus after 40 mg/kg b.w. intravenous administration of 2% Alloxan. One group was kept as diabetic control and in the other three groups was administered 6% aqueous extracts of H. rhamnoides, S. marianum or a combination of the extracts during seven weeks. The stronger effect was obtained in case of H. rhamnoides (Sea buckthorn) extract administration followed by the S. marianum (Milk thistle) extract. The combination of the two extracts proven to have a stronger effect than the extracts given separately.


Alloxan, seabuckthorn, diabetes, milkthistle, rats

Full Text:



Abu-zaiton, A.S., Evaluating the Effect of Silybum marianum Extract on Blood Glucose, Liver and Kidney Functions in Diabetic Rats, Advanced Studies in Biology, 2013, 5, 10, 447-454

Alupului, A., Calinescu, I., Lavric, V., Ultrasonic vs. microwave extraction intensification of active principles from medicinal plants, AIDIC Conference Series, 2009, 09, 1-8, doi:10.3303/ACOS0909001

Ankur, R., Shahjad, A., Alloxan Induced Diabetes: Mechanisms and Effects, International Journal of Research in Pharmaceutical and Biomedical Sciences, 2012, 3, 2, 819-823

Carvalho, E.N., Carvalho, N.A.S., Ferreira, L.M., Experimental model of induction of diabetes mellitus in rats, Acta Cir Bras [serial online] Vol.1 8 Special Edition, 2003. Home page address:

Das, J., Vasan, V., Sil, P.C., Taurine exerts hypoglycemic effect in alloxan-induced diabetic rats, improves insulin- mediated glucose transport signaling pathway in heart and ameliorates cardiac oxidative stress and apoptosis, Toxicol Appl Pharmacol, 2012, 258, 296-308

Etuk, E.U., Animals models for studying diabetes mellitus, Agric Biol J N Am, 2010, 1, 130 - 134

Kruger, D.F., Lorenzi, G.M., Dokken, B.B., Sadler, C.E., Mann, K., Valentine, V., Managing diabetes with integrated teams: maximizing your efforts with limited time, Postgrad Med, 2012, 124, 64-76

Li, W.L., Zheng, H.C., Bukuru, J., De Kimpe, N., Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus, J Ethnopharmacol, 2004, 92, 1–21

Negi, P.S., Chauhan, A.S., Sadia, G.A., Rohinishree, Y.S., Ramteke, R.S., Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts, Food Chem, 2005, 92, 119-124

Pepping, J., Milk thistle: Silybum marianum, Am J Health Syst Pharm, 1999, 56, 1195–1197

Sharma, M., Siddique, M.W., Shamim, A.M., Gyanesh, S., Pillai, K.K., Evaluation of Antidiabetic and Antioxidant Effects of Seabuckthorn (Hippophae rhamnoides L.) in Streptozotocin-Nicotinamide Induced Diabetic Rats, The Open Conference Proceedings Journal, 2011, 2, 53-58

Srinivasan, K., Ramarao, P., Animal models in type 2 diabetes research: an overview, Ind J Med Res, 2007, 125, 451-72

Vaknin, Y., Hadas, R., Schafferman, D., Murkhovsky, L., Bashan, N., The potential of milk thistle (Silybum marianum L.), an Israeli native, as a source of edible sprouts rich in antioxidants, Int J Food Sci Nutr, 2008, 59, 339–346

Velussi, M., Cernigoi, A.M., De Monte, A., Dapas, F., Caffau, C., Zilli, M., Long-term (12 months) treatment with an antioxidant drug (silymarin) is effective on hyperinsulinemia, exogenous insulin need and malondialdehyde levels in cirrhotic diabetic patients, J Hepatol, 1997, 26, 871–879

Wang, B., Lin, L., Ni, Q., Su, C.L., Hippophae rhamnoides Linn. for treatment of diabetes mellitus: A review, Journal of Medicinal Plants Research, 2011, 5, 13, 2599-2607

Zeb, A., Chemical and Nutritional constituents of seabuckthorn juice, Pakistan J Nutr, 2004, 3, 2, 99-106.

Zhang, W., Zhao, J., Wang, J., Pang, X., Zhuang, X., Zhu, X., Qu, W., Hypoglycemic effect of aqueous extract of seabuckthorn (Hippophae rhamnoides L.) seed residues in streptozotocin-induced diabetic rats, Phytotherapy Research, 2010, 24, 2, 228–232

Zhang, W., Zhao, J., Zhu, X., Zhuang, X., Pang, X., Wang, J., Qu, W., Antihyperglycemic effect of aqueous extract of sea buckthorn (Hippophae rhamnoides L.) seed residues in streptozotocin-treated and high fat-diet-fed rats, Journal of Food Biochemistry, 2010, 34, 4, 856–868


  • There are currently no refbacks.

Copyright (c)

Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at]
Phone: +40-256-277160, Fax.: +40-256-277110