Garlic, Cilantro and Chlorella’s Effect on Kidney Histoarchitecture Changes in Cd-intoxicated Prussian carp (Carassius gibelio)

Marioara Nicula, Gabi Dumitrescu, Nicolae Pacala, Lavinia Stef, Camelia Tulcan, Monica Dragomirescu, Ioan Bencsik, Silvia Patruica, Dorel Dronca, Liliana Petculescu Ciochina, Eliza Simiz, Ioan Pet, Adela Marcu, Ioan Caraba

Abstract


Natural chelators from some natural sources have been shown their detox heavy metals ability in human and animals. So the present study was carried out to histological compare the aspect of kidney tissue of Prussian carp’s specimens, subjected to chronic Cd intoxication with and without garlic, cilantro and chlorella dietary supplementation.

150 Prussian carps, with weight of 10-12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water as CdCl2 x ½ H2O), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized cilantro in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed).

The potential protective effect of the three lyophilized products against the impact of cadmium toxicity was evaluated in terms of hystopathological characteristics. For this purpose, fragments of kidney were removed and routinely processed at the end of experimental period and analyzed in light microscopy. A specific QuickPHOTO Micro 2.2 software has been used for the histological study.

Tissue alterations were assessed using the histopathological score ranging from - to +++ depending on the degree and extend of lesions: (-) none, (+) mild occurrence, (++) moderate occurrence, (+++) severe occurrence.

Cd contamination has definitely affected the kidney, inducing severe damage in its structure as: swelling and hypertrophy of tubules with nuclear deterioration, pyknosis and cariorrexis, nucleus and cytoplasm degeneration, capillary ectasia and congestions.

Active compounds from garlic and cilantro powder have shown the most chelating and antioxidant potential, leading to the evident recovery of kidney architecture, while the response at chlorella treatment was less effective than E2 group and without significant difference compared with E3 group.


Keywords


cadmium toxicity; fish; kidney histopathology; lyophilization; natural chelators

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Faculty of Animal Science and Biotechnologies
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
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