The Identification of Histological Changes in Liver and the Evaluation of the Effect of Some Probiotics and Amino Acids on the Invasion of C. jejuni

Lavinia Stef, Liliana Petculescu Ciochina, Ducu Stef, Nicolae Pacala, Dorel Dronca, Adela Marcu, Marioara Nicula, Ioan Pet, Nicolae Corcionivoschi, Gabi Dumitrescu

Abstract


Poultry meat represents a consumer food worldwide and can be a source of pathogen agents (Salmonella enterica spp., Escherichia coli, and Campylobacter jejuni). Therefore, the identification of the best methods to reduce the infections of these pathogens from poultry meat represents a critical aspect for producers. The purpose of this study was to identify the histological changes in the liver and to evaluate the influence of some probiotic strains and of some amino acids in the invasion of Campylobacter jejuni. The biological material was represented by poultry broilers, the hybrid ROSS 308, which were allocated randomly in seven experimental groupss, of 10 individuals/group that were treated as follows: G0 (control); G1 - L. paracasei CMGB 18 and L. rhamnosus CMGB 34 (0-42 days); G2 - L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32 (0-42 days); G3 - L. paracasei CMGB 18 and L. rhamnosus CMGB 34 (35-42 days); G4 - L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32 (35-42 days); G5 - L. paracasei CMGB 18, L. rhamnosus CMGB 34, L Treonine and DL Methionine (0-42 days); G6 - L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31, L. lactis CMGB 32, L Treonine and DL Methionine (35-42 days). The microscopic aspects pointed out by us in this study suggest the pathogenicity of C. jejuni, marked by the appearance of  inflammatory areas, with the presence of a perivascular and diffuse leukocytes infiltration and, also, presence of pyknotic nuclei. Moreover, the results show the beneficial effect of the probiotic strains L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32, with the reduction of the changes caused by C.jejuni and the production of toxins, thus preventing the damage of the hepatic cells. The most powerful effect was noticed when all the four probiotic strains were administrated, throughout the experiment 0-42days.


Keywords


C. jejuni, chicken broilers, liver, probiotic, histological changes

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References


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