Gross Morphology Analysis of Osteoarthritis in Rabbits

Svätoslav Hluchý

Abstract


The aim of this study was to evaluate the effect of glucosamine sulfate on morphological changes investigated on the articular cartilage of knee joint during development of osteoarthritis in rabbits following collagenase-induced osteoarthritis. A total of 32 New Zealand White male and female rabbits were used in this study. All animals were kept in standard conditions at Research Institute of Livestock Production in Nitra. Animals were divided into 4 groups: healthy control group, osteoarthritic untreated group and two experimental groups. After collagenase-induced osteoarthritis daily oral administration of glucosamine sulfate ( 80 mg.kg-1  and 160mg.kg-1 of live weight) was performed.  At the end of the eight week, the rabbits were sacrificed and their both right and left knees with proximal femur and distal tibia were harvested. Gross morphology of knee joint was tested. Basal statistical indicators were calculated from obtained data using Statgraphics software and analysis of variance. Morphological changes in osteoarthritic group without treatment were seen on both, medial and lateral region, but markedly on medial condyle. Articular cartilage was characterized by a rough and hypertrophic appearance with severe erosions. The severity of cartilage damage was generally lower in glucosamine treated groups in comparison with osteoarthritis group. The gross morphological examination of healthy control revealed very little or no changes. Gross morphological grading of cartilage damage showed significantly lower extent of damage in glucosamine treated groups.


Keywords


Gross morphology, knee joint, osteoarthritis, rabbit, glucosamine sulfate

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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
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Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110