Antiproliferative Effects of Tetrabuthylammonium Chloride Ionic Liquid on HCT 8 Human Colon Carcinoma Cells

Gabi Dumitrescu, Roxana Popescu, Marioara Nicoleta Filimon, Doina Verdeș, Nicolae Păcală, Ioan Bencsik, Dorel Dronca, Mihai Mituletu, Liliana Petculescu Ciochină


The ionic liquids have attracted a great of attention in the scientific community due to their potential pharmaceutical such as antimicrobial. In this paper, the main objective was the assessment of the cytotoxic effect of tetrabutylammonium chloride against HCT 8 human colon carcinoma cell line. The cells were cultured in 75 cm2 culture flasks  using RPMI medium supplemented with 10% inactivated fetal bovine serum (FBS), penicillin (100 IU/mL) and streptomycin (100 μg/mL) and maintained at 37 °C and 5% CO2. Before achieving viability test, the cells were harvested using trypsin solution (0.25%). Then, the cells were seeded in 24 – well plates at a density of 5 x 105 cells/mL in 100 µL medium/well in order to reach confluence. After 24 h, the medium was replaced with fresh medium containing different concentrations of ionic liquid, respectively, 0.085, 0.17, 0.34, 0.68 and 1.36 mg /mL. Control group contained cells without treatment. Cell proliferation kinetics have been studied at 24 and 48 h after IL treatment, following trypsinization and counting total cells per plate by using a Trypan blue dye and a hemocytometer. Data obtained from the growth kinetics assay shows that the tetrabutylammonium chloride (TBAC) had an inhibitory effect on the growth of cells in a concentration dependent manner. The maximum inhibitory effect on HCT 8 cells it was obtained at 1.36 mg TBAC/mL.


Ionic liquids, HCT 8 human colon carcinoma cell line, antiproliferative effect

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Pernak, J., Sobaszkiewicz, K., Mirska, I., 2003. Anti-microbial activities of ionic liquids. Green Chem., 5, 52–56.

Couling, D.J., Bernot, A.R., Docherty, K.M., Dixon, J.K. & Magin, E.J., 2006. Assessing the factors responsible for ionic liquid toxicity to aquatic organisms via quantitative structure – property relationship modeling. Green Chem. 8, 82-90

Alfassi, Z. B., Huie R. E., Milman B. L., and Neta P., 2003. Electrospray ionization mass spectrometry of ionic liquids and determination of their solubility in water. Anal. Bioanal. Chem. 377, 159Ð164

Sheldon, R.A., 2005. Green solvent for sustainable organic synthesis: State of the art. Green Chem. 7, 267-278.

Ranke J, Molter K., Stock F., Bottin-Weber V., Poczobutt, J., Hoffman J., Ondruscka B., Filser J. & Jastorf B., 2005. Biological effects of imidazolium ionic liquids with varying in acute Vibrio fischeri and WST – 1 cell viability assay. Ecotoxicology and Environmental Safety. 60 (3), 350.

Yu, M., Li, S.M., Li, Zhang, X-Y., B-J. & Wang, J.J., 2009. Acute effects of 1-octyl-3-methilimidazolium bromide ionic liquid on the antioxidant enzyme system of mouse liver. Ecotox. Environ. Saf. 71, 903-906.

Attri, P., Venkatesu, P., 2012. Influence of protic ionic liquids on the structure and stability of succinylated Con A. Int. J. Biol. Macromol., 51, 119–128.

Hough-Troutman, W.L., Smiglak, M., Griffin, S., Reichert, W.M., Mirska, I., Jodynis-Liebert, J., Adamska, T., Nawrot, J., Stasiewicz, M., Rogers, R.D. et al., 2009. Ionic liquids with dual biological function: Sweet and anti-microbial, hydrophobic quaternary ammonium-based salts. New J. Chem. 33, 26–33

Pernak, J Goc, I., Mirska, I., 2004. Antimicrobial., activities of protic ionic liquids with lactate anion. Green Chem., 6, 323–329.

Kumar, V., Malhotra, S.V., 2009. Study on the potential anti-cancer activity of phosphonium and ammonium-based ionic liquids. Bioorg. Med. Chem. Lett., 19, 4643–4646.

Stock, F., Hoffman, J., Ranke, J., Ondruschka, B., Jastorff, B., 2004. Effects of ionic liquids on the acetylcholinesterase—A structure–activity relationship consideration. Green Chem., 6, 286–290.

Skladanowski, A.C., Stepnowski, P., Kleszczynski, K., Dmochowska, B., 2005. AMP deaminase in vitro inhibition by xenobiotics A potential molecular method for risk assessment of synthetic nitro- and polycyclic musks, imidazolium ionic liquids and N-glucopyranosyl ammonium salts. Environ. Toxicol. Pharmacol., 19, 291–296

Hough, W.L.,Smiglak, M., Rodrıguez, H., Swatloski, R.P., Spear, S.K., Daly, D.T., Pernak, J., Grisel, J.E., Carliss, R.D., Soutullo, M.D.; et al., 2007. The third evolution of ionic liquids: Active pharmaceutical ingredients. New J. Chem. 31, 1429–1436.

Malhotra, S.V., Kumar, V., 2010. A profile of the in-vitro antitumor activity of imidazolium-based ionic liquids. Bioorg. Med. Chem. Lett., 20, 581–585.


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