Histomorphometric Features of Deltoid Muscle (Musculus Deltoideus) in the Mouflon (Ovis ammon musimon Pal.) and Domestic Sheep

Nenad Nedeljkovic, Gabi Dumitrescu, Liliana Petculescu Ciochină, Ioan Bencsik, Dorel Dronca, Eliza Simiz, Marioara Nicula, Adela Marcu, Valeriu Carabă, Iancu Tiberiu, Nicolae Pacală

Abstract


The aim of this study was to determine histomorphometry of the skeletal muscle at rams from the Tigaie breed and males mouflon (Ovis ammon musimon Pal.) knowing that, in addition to a variety of factors, both the fiber thickness and muscle fascicles influence of meat quality. The study was conducted on the deltoid muscle fragments taken from five individuals of the each species. The muscle samples were processed according to the method of embedded in paraffin, sectioned to a thickness of 5 µm and stained with Mallory's trichrome method. From the histomorphometric analysis resulted that in mouflon, the average area of muscle fascicles is larger, as compared with the ram (p <0.05), the ratio between the fascicles muscle area and the interfascicular connective tissue being 2.939:1 to mouflon and 1,624:1 to ram. Also, at mouflon the muscle fibers are thicker, the area and perimeter  registering an average value of 486.60 μm2, respectively 82.83 μm while in the ram, the area of the muscle fibers is 281.56 μm2 and the perimeter of 62.92 μm (p <0.001). The amount of interfibrillar connective tissue is higher in the ram (p <0.05) and the ratio between the muscular fibers area and interfibrillar connective tissue area was 1.649:1 in mouflon and 1.451:1 at ram (p<0.05).


Keywords


Ovis ammon musimon Pal., ram, Musculus deltoideus, muscle histomorphometry

Full Text:

PDF

References


Melton, S.L., 1990. Effect of flavour of red meat: a review. J. Anim. Sci. 68, 4421-4435

Wood, J., M. Enser, 1997. Factor influencing fatty acids in meat and the role of antioxidants in improving meat quality. Brit. J. Nutr., 78: 549 – 560.

Grundy, S.M., 1987. Monounsaturated fatty acids, plasma cholesterol, and coronary heart disease. Am. J. Clin. Nutr. 45, 1168-1175.

Williams, C.M., 2000. Dietary fatty acids and human health. Annales de Zootechnie, 49, 165-180

De Smet, S., K. Raes, D. Demeyer, 2004. Meat fatty acid composition as affected by fatness and genetic factors: a review. Anim. Res. 53, 81-98.

Wood, J.D., Richardson, R.I., Nute, G.R., Fisher, A.V., Campo, M.M., Kasapidou, E., Sheard, P.R., Enser, M., 2003. Effects o fatty acids on meat quality: a review. Meat Science, 66: 21-32.

Enser, M., K. G. Hallett, B. Hewitt, G. A. Fursey, J. D. Wood, G. Harrington, 1998. Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Sci. 49, 329-341.

Hoffman, L.C., Kroucamp, M., Mauley, M., 2007. Meat quality characteristics of springbok (Antidorcas marsupialis), 3: fatty acid composition as influenced by age, gender and production region. Meat Sci 76: 768-773.

Nikolina Kelava Ugarovic, Damir Ugarovic, 2013. Chemical and fatty acid composition of male mouflon (Ovis ammon musimon Pal.) meat. Eur. J. Wildl Res, 59: 469-475.

Nenad Nedeljkovic, Nicolae Pacală, Liliana Petculescu Ciochină, Ioan Bencsik, Dorel Dronca, Eliza Simiz, Adela Marcu,Valeriu Carabă, Gabi Dumitrescu, 2016. Histomorphometric of deltoid muscle (Musculus Deltoideus) in the hybrids of mouflon (Ovis ammon musimon Pal.) and domestic sheep. Scientific Papers, Animal Science and Biotechnologies, 49(1).

Bancroft, J. D. and Stevens, A. 1990. Theory and Practice of Histological Tehniques. 3rd edn. Churchill, Livingstone, pp. 117-118.

Winkelmayer, R., Lebersorger, P., Zedka, H. F., Forejtek, P., Vodňansky, M., Večerek, V. et al., 2005: Hygiene of Game Meat (In Czech). Central European Institute of Animal Ecology, Wien-Brno-Nitra, 168 pp.

Hulland, T.J., 1981. Histochemical and Morphometric Evaluation of Skeletal Muscle of Cachectic Sheep. Vet Pathol, 18: 279-298.

Peinado, B., Latorre, R., Vaquez-Auton, J.M., Poto, A., Ramirez, G., Lopez-Alborz, O., Moreno, F., Gil, F., 2004. Histochemical skeletal muscle fibre types in the sheep. Anat. Histol. Embryol., 33: 236-243.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Scientific Papers Animal Science and Biotechnologies


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110