ASSESSMENT OF SELECTED CHEMICAL COMPONENTS IN DIFFERENT TYPES OF CHOCOLATES

Alica Bobková

Abstract


The aim of the study was observed selected and compared chemical components in commercial and raw chocolates. HPLC was used to determine Galic acid, Theobromin, Catechin, Caffeine, Epicatechin and Epigallocatechin 3 gallate in 17 commercial chocolates.  Result shows very strong inverse correlation between Epigallocatechin 3 gallate and Galic acid. There was also observed strong positive correlation between Theobromin and Caffeine, Epicatechin and Galic acid, Epigallocatechin 3 gallate and Theobromin,  Caffeine and Epigallocatechin 3 gallate. The PCA revealed that 78.83 % of the total variation embodied in five variables could be effectively condensed into and explained by first two principal components (PCs), with eigenvalues of 3.00 and 1.72, respectively.  The PC1, accounting for 50.05 % of the inertia, contrasted Galic acid and Caffeine, Epigallocatechin 3 gallate and Theobromin whereas PC2, explaining 28.77 % of the inertia, clearly reflected the different content of Epigallocatechin 3 gallate and Epicatechin. The total variability depends on the tested chemical parameters. The tested raw chocolates were characterized in particular by the caffeine parameters Epigallocatechin 3 gallate.


Keywords


Chocolate, HPLC, PCA

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References


Alañón M, Castle S, Siswanto P, Cifuentes-Gómez T, Spencer J. Assessment of flavanol stereoisomers and caffeine and theobromine content in commercial chocolates. Food Chemistry. 2016, pp. 177-184. doi:10.1016/j.foodchem.2016.03.116.

Bobková A, Šnirc M, Fikselová M. Selected parameters of Arabica coffee quality affected by its geographical origin. Potravinarstvo Slovak Journal of Food Sciences. 2017, pp. 260-265. doi: https://dx.doi.org/10.5219/794

Fisher N, Hughes M, Gerhard-Herman M, Hollenberg N. Flavanol-rich cocoa induces nitric-oxide-dependent vasodilation in healthy humans. Journal of Hypertension. 2003, pp. 2281-2286. doi:10.1097/00004872-200312000-00016.

Heiss C. Vascular Effects of Cocoa Rich in Flavan-3-ols. JAMA: The Journal of the American Medical Association. 2003, pp. 1030-1031. doi:10.1001/jama.290.8.1030.

Ostertag L, Kroon P, Wood S et al. Flavan-3-ol-enriched dark chocolate and white chocolate improve acute measures of platelet function in a gender-specific way-a randomized-controlled human intervention trial. Molecular Nutrition & Food Research. 2012, pp. 191-202. doi:10.1002/mnfr.201200283.

Schinella G, Mosca S, Cienfuegos-Jovellanos E et al. Antioxidant properties of polyphenol-rich cocoa products industrially processed. Food Research International. 2010, pp. 1614-1623. doi:10.1016/j.foodres.2010.04.032.

Hatano T, Miyatake H, Natsume M et al. Proanthocyanidin glycosides and related polyphenols from cacao liquor and their antioxidant effects. Phytochemistry. 2002, pp. 749-758. doi:10.1016/s0031-9422(02)00051-1.

Andújar I, Recio M, Giner R, Ríos J. Cocoa Polyphenols and Their Potential Benefits for Human Health. Oxidative Medicine and Cellular Longevity. 2012, pp. 1-23. doi:10.1155/2012/906252.

Aculey P, Snitkjaer P, Owusu M et al. Ghanaian Cocoa Bean Fermentation Characterized by Spectroscopic and Chromatographic Methods and Chemometrics. Journal of Food Science. 2010, pp. S300-S307. doi:10.1111/j.1750-3841.2010.01710.x.

Batista N, de Andrade D, Ramos C, Dias D, Schwan R. Antioxidant capacity of cocoa beans and chocolate assessed by FTIR. Food Research International. 2016, pp. 313-319. doi:10.1016/j.foodres.2016.10.028.

Wollgast J, Anklam E. Polyphenols in chocolate: is there a contribution to human health?. Food Research International. 2000, pp. 449-459. doi:10.1016/s0963-9969(00)00069-7.

Nakagawa K, Miyazawa T. Chemiluminescence–High-Performance Liquid Chromatographic Determination of Tea Catechin, (−)-Epigallocatechin 3-Gallate, at Picomole Levels in Rat and Human Plasma. Analytical Biochemistry. 1997, pp. 41-49. doi:10.1006/abio.1997.2098.

El-Shahawi M, Hamza A, Bahaffi S, Al-Sibaai A, Abduljabbar T. Analysis of some selected catechins and caffeine in green tea by high performance liquid chromatography. Food Chemistry. 2012, pp. 2268-2275. doi:10.1016/j.foodchem.2012.03.039.

Lee K, Kim Y, Lee H, Lee C. Cocoa Has More Phenolic Phytochemicals and a Higher Antioxidant Capacity than Teas and Red Wine. Journal of Agricultural and Food Chemistry. 2003, pp. 7292-7295. doi:10.1021/jf0344385.


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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

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