Glucose Release During in Vitro Digestion of Wheat

Rodica Căpriță, Adrian Caprita

Abstract


The research investigated the dynamics of released glucose from whole wheat flour during in vitro gastric and intestinal digestion. Two experiments were conducted. Experiment 1 (gastric digestion) was an enzymatic hydrolysis with a pepsin solution at pH 2.0 and 37°C for 120 minutes. In experiment 2, first step was followed by the hydrolysis with the multi-enzyme pancreatin (intestinal digestion), at pH 6.8 and 37°C for 4 h. The released glucose (RG) and the refractive index (RI) were determined from the supernatant obtained after centrifugation. Experiments showed an initial increase in the glucose released from 1 g wheat up to 0.472 mg/mL after 1 h of gastric digestion, then followed by decrease. The lowest value was recorded after 2 hours of gastric digestion (0.332 mg/mL). The concentration of the reducing sugars increased throughout the intestinal digestion from 0.549 mg/mL up to 1.990 mg/mL, as a result of starch hydrolysis to dextrins, maltose and then to glucose. The high positive correlation between RG and RI suggests that RI may be used as a simple and rapid method for estimating the released glucose during in vitro digestion.


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References


Lineback, D. R., Rasper, V. F., Wheat carbohydrates, in: Wheat: Chemistry and Technology, vol. 1, ed. by Pomeranz Y. Am. Assoc. Cereal Chem., St. Paul, 1988, pp. 277-371.

Knudsen, K. E., Fiber and nonstarch polysaccharide content and variation in common crops used in broiler diets, Poultry Science, 2014, 93, 2380–2393.

Căpriţă, R., Căpriţă, A., Chemical methods for the determination of soluble and insoluble non-starch polysaccharides-Review, Anim. Sci. Biotechnol., 2000, 44, 73-80

Schweizer, T. F., Würsch, P., Analysis of dietary fiber, in: The Analysis of Dietary Fiber in Food, ed. by James WPT and Theander O. Marcel Dekker, New York, 1981, pp. 203-216.

Caprita, A., Caprita, R., The effect of thermal processing on soluble dietary fibre fraction in wheat, Journal of Food, Agriculture & Environment, 2011, 9, 14-15.

Choct, M., Enzymes for the feed industry: past, present and future, World Poultry Sci. J., 2006, 62: 5-16.

Dervilly, G., Saulnier, L., Roger, P., and Thibault, J.-F., Isolation of homogeneous fractions from wheat water-soluble arabinoxylans. Influence of the structure on their macromolecular characteristics, Journal of Agricultural and Food Chemistry, 2000, 48, 270-278/

Saeed, F., Pasha, I., Anjum, F. M., and Sultan, M. T., Arabinoxylans and arabinogalactans: a comprehensive treatise, Journal of Food Science and Nutrition, 2011, 51, 467-476.

Hartmann, G., Michael, P., and Peter, K., Isolation and chemical characterization of water-extractable arabinoxylan from wheat and rye during bread making, European Food Research and Technology, 2005, 221, 487–492.

Mares, D. J., Stone, B. A., Studies on wheat endosperm. I. Chemical composition and ultra structure of the cell walls, Australian Journal of Biological Sciences , 1973, 26, 793–812.

Caprita, R., Caprita, A., Julean, C., Pandur, C., Drinceanu, D., and Stef L., Relationship between feed water extract viscosity and intestinal viscosity in poultry, J. Biochem., 2010, 150, 199.

Choct, M., Annison, G., The inhibition of nutrient digestion by wheat pentosans, Br. J. Nutr., 1992, 67,123-132.

Pettersson, D., Aman, P., Enzyme supplementation of a poultry diet containing rye and wheat, Br. J. Nutr., 1989, 62, 139-149.

Fengler, A. I., Marquardt, R. R., Water-soluble pentosans from rye: II. Effects on rate of dialysis and retention of nutrients by the chick, Cereal Chem., 1988, 65, 298-302.

Edwards, C. A., Johnson, I. T., and Read, N. W., Do viscous polysaccharides slow absorption by inhibiting diffusion or convection?, Eur. J. Clin. Nutr., 1988, 42, 306–312.

Ikegami, S., Tsuchihashi, F., Harada, H., Tsuchihashi, N., Nishide, E., and Innami ,S., Effect of viscous indigestible polysaccharides on pancreatic-biliary secretion and digestive organs in rats, J. Nutr., 1990, 120, 353–360.

Salih, M. E., Classen, H. L., and Campbell, G. L., Response of chickens fed hull-less barley to dietary beta-glucanases at different ages, Anim. Feed Sci. Tech., 1991, 33, 139-149.

Feighner, S. D., Dashkevicz, M. P., Effect of dietary carbohydrates on bacterial cholyltaurine hydrolase in poultry intestinal homogenates, Appl. Environ. Microbiol., 1988, 54, 334-342.

Malunga, L. N., Eck, P., and Beta, T., Inhibition of Intestinal α-Glucosidase and Glucose Absorption by Feruloylated Arabinoxylan Mono- and Oligosaccharides from Corn Bran and Wheat Aleurone. J. Nutr. Metab., 2016, 1-9.

Foster-Powell, K., Holt, S. H., J. C. Brand-Miller, J. C., International table of glycemic index and glycemic load values, The American Journal of Clinical Nutrition, 2002, 76, 5–56.

Ludwig D. S., Eckel, R. H., The glycemic index at 20y, The American Journal of Clinical Nutrition, 2002, 76, 264S–265S.

Jenkins, D. J. A., Wolever, T. M. S., Taylor, R. H., Barker, H. M., Fielden, H., and. Gassull, M. A., Lack of effect of refining on the glycemic response to cereals, Diabetes Care, 1981, 4, 509–513, 1981.

Belobrajdic, D. P., Bird, A. R., The potential role of phytochemicals in wholegrain cereals for the prevention of type-2 diabetes, Nutrition Journal, 2013, 12, article 62.

Boisen, S., Fernández, J. A., Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses, Anim. Feed Scim Tech., 1997, 68, 277–286.

Li, W. F., Sun, J. Y., and Xu, Z. R., Effects of NSP degrading enzyme on in vitro digestion of barley, Asian-Aust. J. Anim. Sci., 2004, 17, 122-126.

Căpriţă, R., Căpriţă A., The Variation of relative and reduced viscosity with concentration in wheat and barley aqueous extracts, Lucrări Ştiinţifice, Zootehnie şi Biotehnologii/Scientific Papers: Animal Science and Biotechnologies, 2012, 54(2), 117-120.


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LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

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