Microorganisms isolated from the Slovak traditional cheese “Parenica”and their identification with mass spectrometry

Miroslava Kačániová


Cheese is one of the most important products of dairy industry from the economic and nutritional points of view that demands the high processing technology and production hygiene. The aim of the study was to evaluate microbiological quality of traditional Slovak cheese “Parenica” produced from sheep milk. The coliforms, enterococci, lactic acid bacteria and microscopic filamentous fungi were detected in cheese samples. MALDI-TOF MS was applied for identification of isolates. In group of lactic acid bacteria three genera were identified: Lactococcus and Leuconostoc. Lactobacillus spp. was the most abundant genus, which was found in 25, Leuconostoc in 10 and Lactococcus in 7 samples. Lactobacillus was mostly represented with four species: Lactobacillus plantarum, L. acidophilus, L. ultunensis, L. casei. Another bacterial isolates included Gram-positive and Gram-negative Enterococcus faecium, Streptococcus, Raoutella ornithinolytica and Klebsiela oxytoca. Our study shows that MALDI-TOF MS could serve as an important method for identifying microorganisms for dairy industry needs.


cheese, microorganisms, identification, mass spectrometry

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Corfield, A.P., Carroll, D., Myerscough, N., Probert, C.S., Mucins in the gastrointestinal tract in health and disease, Journal of Frontiers in Bioscience, 2001, 6, 321-1357

Settanni, L., Corsetti, A., Application of bacteriocins in vegetable food biopreservation, International Journal of Food Microbiology, 2008, 121, 123-138

Hernandez, D., Cardell, E., Zarate, V., Antimicrobial activity of lactic acid bacteria isolated from Tenerife cheese: initial characterization of plantaricin TF711, a bacteriocin-like substance produced by Lactobacillus plantarum TF711, Journal of Food Microbiology, 2005, 99: 77-84

Nielsen, D.S., Schillinger, U., Franz, C.M.A.P., Bresciani, J., Amoa-Awua, W., Holzapfel, W.H., Jakobsen, M., 2007. Lactobacillus ghanensis sp. nov., a motile lactic acid bacterium isolated from Ghanaian cocoa fermentations, International Journal of Systematic and Evolutionary Microbiology, 2007, 57, 1468–1472

Chao, S.H., Tomii, Y., Sasamoto, M., Fujimoto, J., Tsai, Y.C., Watanabe, K., Lactobacillus capillatus sp. nov., a motile Lactobacillus species isolated from stinky tofu brine, International Journal of Systematic and Evolutionary Microbiology, 2008, 58, 2555-2559

Ogier, J.C., Son, O., Gruss, A., Tailliez, P., Delacroix-Buchet, A., Identification of the Bacterial Microflora in Dairy Products by Temporal Temperature Gradient Gel Electrophoresis, Applied and environmental microbiology, 2002, 3691-3701

Leclerc, H., Mossel, D.A,. Edberg, S.C., Struijk, C.B., Advances in the bacteriology of the coliform group: Their suitability as markers of microbial water safety, Annual Review Microbiology, 2001, 55, 201-234

Coton, M., Delbés-Paus, C., Irlinger, F., Desmasures, N., Le Fleche, A., Stahl, V., Montel, M.C., Coton, E., 2012. Diversity and assessment of potential risk factors of gram-negative isolates associated with French cheeses, Food Microbiology, 2012, 29, 88-98

Quigley, L., O'Sullivan, O., Stanton, C., Beresford, T.P., Ross, R.P., Fitzgerald, G.F., Cotter, P.D., The complex microbiota of raw milk, FEMS Microbiology Review, 2013, 37, 664-698

Masiello, S.N., Martin, N.H. Trmčič, A. Wiedmann, M., Boor, K.J., Identification and characterization of psychrotolerant coliform bacteria isolated from pasteurized fluid milk, Journal of Dairy Sciences, 2016, 99, 130-140

Edberg, S.C., Rice, E.W., Karlin, R.J., Allen, M.J., Escherichia coli: The best biological drinking water indicator for public health protection, Symposium Series Society for Applied Microbiology, 2000, 29, 106S-116S

Stevens, M., Ashbolt, N., Cunliffe. D., Review of coliforms as microbial indicators of drinking water quality. Recommendations to change the use of coliforms as microbial indicators of drinking water quality. Australian Government, National Health and Medical Research Council. Biotext Pty Ltd., Canberra, Australia. 2003

Paruch, A.M., Maehlum, T., Specific features of Escherichia coli that distinguish it from coliform and thermotolerant coliform bacteria and define it as the most accurate indicator of faecal contamination in the environment, Ecological Indicators, 2012, 23, 140-142

Buszewski, B., Rogowska, A., Pomastowski, P., Złoch, M., Railean-Plugaru, V., Identification of microorganisms by modern analytical techniques, Journal of AOAC International, 2017, 100(6), 1607-1623

Atasever, M., Ucar, G., Keles, A. Kose, Z., The effects of natural smoke and liquid smoke applications on the quality factors of Kashar cheese, Turkish Journal of Animal Science, 2003, 27, 781-787

Yucel, A., Ozcan, T., Food Hygiene and Sanitation pp. 123. Bursa: Uludag University, Hardcopy Lecture Notes. 2010, 36

Uysal, H., Kavas, G., Kesenkas, H., Akbulut, N., Some properties of traditional circassian cheese produced in Turkey, International Journal of Dairy Science, 2010, 5, 150-152

Ozcan, T., Akpinar-Bayizit, A., Sahin, O.I. Yilmaz-Ersan, L., The formation of polycyclic hydrocarbons during smoking process of cheese, Mljekarstvo, 2011, 61 193-198

Van Kessel, J.S., Karns, J.S., Gorski, L., McCluskey, B.J., Perdue, M.L., Prevalence of Salmonellae, Listeria monocytogenes and fecal coliforms in bulk tank milk on US dairy farms, Journal of Dairy Sciences, 2004, 87, 2822-2830

Boor, K.J., Brown, D.P., Murphy, S.C., Kozlowski S.M., Bandler, D.K., Microbiological and chemical quality of raw milk in New York State, Journal of Dairy Sciences, 1998, 81, 1743-1748

Jayarao, B.M., Wang, L., A study on the prevalence of gram-negative bacteria in bulk tank milk, Journal of Dairy Sciences, 1999, 82, 2620-2624

D’Amico, D.J., Groves, E., Donnelly, C.W., Low incidence of foodborne pathogens of concern in raw milk utilized for farmstead cheese production, Journal of Food Protection, 2008, 71, 1580-1589

Jackson, E.E., Erten, E.S., Maddi, N., Graham, T.E., Larkin, J.W., Blodgett, R.J., Detection and enumeration of four foodborne pathogens in raw commingled silo milk in the United States, Journal of Food Protection, 2012, 75, 1382-1393

Kagkli, D.M., Vancanneyt, M., Vandamme, P., Hill, C., Cogan, T.M., Contamination of milk by enterococci and coliforms from bovine faeces, Journal of Applied Microbiology, 2007, 103, 1393-1405

Hogan, J., Smith, K.L., Coliform mastitis, Veterinary Research, 2003, 34, 507-519


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