Lyophilisation of lactic bacteria with probiotic effect for production of starter cultures

Teodor Vintilă, Camelia Sava, Daniela Moț, Eniko Gaspar, Horia Barbu, Călin Julean

Abstract


Selection of lactic acid bacteria (LAB) for probiotic effect and preservation of LAB by lyophilized have been studied. The two LAB strains belonging to Lactobacillus plantarum and Lactobacillus pentosus producing bacteriocins in inhibitory concentration for colibacilli were selected from a total of 17 tested strains. The selected strains were further tested for their ability to survive the preservation process by lyophilisation. Two methods of preserving LAB by lyophilisation were tested and the viability of cells in lyophilized products was monitored in order to obtain products used as starter cultures. The presence of inhibition zones in mixt cultures indicates the ability of LAB to inhibit the growth of E. coli bacteria due to the synthesis of bacteriocins. During three years period, the viability of lyophilized LAB was monitored. Obtained data indicate that starter cultures of LAB can be stored in the lyophilized state for at least three years in dark and cool place, and the inoculation rate can be increased after this period by 50% if the starter culture was preserved and stored by applying the described techniques.

Keywords


lactic acid bacteria, probiotic, preservation, lyophilisation

Full Text:

PDF

References


Stoica I., Vassu Dimov T., Săsărman E., Biologia și taxonomia moleculară a microorganismelor - Colecția de culturi microbiene, Editura Arvin Press.

Săsărman Elena, Jurcoane Ștefania- Microbiologie industrială, Editura Mirton, Timișoara, 2000

Berg. R. D., The indigenous gastrointestinal microflora.,2002, Trends Microbiol 4:430-435

Vintilă T., Dinu L. D., Tehnologia Produselor de Biosinteză, Manual de laborator, Editura Orizonturi Universitare, Timişoara, 2004. ISBN 973-638-156-0

Simione F.P., Brown E.M., ATCC Preservation Methods: Freezing and Freeze-Drying (2nd ed.), ATCC, Rockville, MD (1991)

Vintilă T., Microbiologie Aplicată: lucrări practice de laborator, Editura Agroprint, Timişoara, 2015.

Vintilă T., Săsărman E., Mic A., Studii privind conservarea în azot lichid a bacteriilor lactice, Sesiunea Ştiinţifică Anuală a FZB, Timişoara, 1999, pag. 467-470

Bizani, D., Brandelli, A. Influence of media and temperature on bacteriocin production by Bacillus cereus 8A during batch cultivation. Appl Microbiol Biotechnol 65, 158–162 (2004).

Russell, J. B., and H. C. Mantovani 2002, The bacteriocins of ruminal bacteria and their potential as an alternative to antibiotics. J Mol Microbiol Biotechnol 4:347-355

Shu Q., and H. S. Gill. Immune protection mediated by the probiotic Lactobacillus rhamnosus HN001 (DR20) against Escherichia coli O157:H7 infection in mice. FEMS Immunol Med Microbiol 34:59-64, 2002

Sakamoto, I., Igarashi, K. Kimura, A. Takagi, T. Miwa, and Y. Koga, 2001. Suppressive effect of Lactobacollus gasseri OLL 2716 (LG21) on Helocobacter pylori infection in humans. J Antimicrob Chemother 47:709-710

Corcionivoschi N., Drinceanu D., Probioticele la timpul prezent, 2009, Editura Mirton.

Dupuis, C., C. Corre, and P. Boyaval, 1995, Proteinase Activity of Dairy Propionibacterium. Applied Microbiology and Biotechnology 42:750-755

Perdigon, G., S. Alvarez, M. Rachid, g. Aguero, and N. Gobbato. 1995, Immune system stimulation by probiotics. J Dairy Sci 78:1597-1606

Salminen, S., and A. V. Wright, Lactic Acid Bacteria. Microbiology and Functional Aspects. New York, 2000.

Vintilă Daniela, T. Vintilă, D. Dronca, Obtaining and conditioning lactic bacteria inoculants using bentonite, Bulletin of USAMV Cluj-Napoca, Animal Husbandry and Biotechnologies, Vol. 62/2006, 238-242.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Scientific Papers Animal Science and Biotechnologies


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110