Aflatoxin in Maize Silage Collected from AP Vojvodina, Serbia
Keywords:
aflatoxin, AP Vojvodina, maize silage, SerbiaAbstract
The aim of this work was to investigate aflatoxin (AF) occurrence in maize silage samples from 2017 harvest. In total, 30 samples of maize silage (whole plant) for nutrition of milking cows were inspected for AF occurrence. The samples originated from three regions (Bačka, Banat and Srem) in autonomous province (AP) of Vojvodina, Republic of Serbia (10 samples collected from each region). In AP Vojvodina, overall AF presence was 67% with its levels ranged from 3.52 to 24.64 µg/kg. Levels were standardized to 12% moisture content in all samples. Regarding each region, the highest presence was observed in Srem (80%) followed by Banat (70%), while the lowest presence was found in Bačka region (50%). However, the average AF contamination levels were not correlated to its presence. The highest AF level of 12.76 µg/kg (ranged from 6.16 to 24.64 µg/kg) was found in Srem region, whereas its lowest level was observed in Banat region (average of 4.40 µg/kg, ranged from 3.52 to 5.28 µg/kg). In the region of Bačka the average AF level was 8.45 µg/kg. Despite high presence, none of the samples contained AF above maximum level set by Serbian regulation (30 µg/kg), while 2 samples from Srem region exceeded the EU maximum level of 20 µg/kg. These results may be a result of warm and dry weather conditions during summer months of 2017, that prevailed on almost entire territory of AP Vojvodina.
References
USDA, Romania’s Aflatoxin Problem in Dairy Proving Toxic, RO1312, 2013.
Masoero, F., Gallo, A., Moschini, M., Piva, G., and Diaz, D., Carryover of Aflatoxin from Feed to Milk in Dairy Cows with Low or High Somatic Cell Counts, Animal, 2007, 1 (9), 1344–50.
Britzi, M., Friedman, S., Miron, J., Solomon, R., Cuneah, O., Shimshoni, J. A., Soback, S., Ashkenazi, R., Armer, S., and Shlosberg, A., Carry-over of aflatoxin B1 to aflatoxin M1 in high yielding Israeli cows in mid-and late-lactation, Toxins, 2013, 5 (1), 173-183.
Commission Regulation (EC) No 1881/2006 of 19 December 2006 Setting Maximum Levels for Certain Contaminants in Foodstuffs, Official Journal of the European Union, 2006, L364, 5-24.
Serbian Regulation, Maximum allowed contents of contaminants in food and feed, Official Gazette of the Republic of Serbia, 2011, 28.
Serbian Regulation, Maximum allowed contents of contaminants in food and feed, Official Gazette of the Republic of Serbia, 2013, 20.
Serbian Regulation, Amendment on Serbian regulation 2011 “Maximum allowed contents of contaminants in food and feed”, Official Gazette of the Republic of Serbia, 2014, 39.
Serbian Regulation, Amendment on Serbian regulation 2011 “Maximum allowed contents of contaminants in food and feed”, Official Gazette of the Republic of Serbia, 2014, 72.
Völkel, I., Schröer-Merker, E., and Czerny, C. P., The carry-over of mycotoxins in products of animal origin with special regard to its implications for the European food safety legislation, Food and Nutrition Sciences, 2011, 2, 852-867.
Cheli, F., Campagnoli, A., and Dell’Orto, V., Fungal populations and mycotoxins in silages: From occurrence to analysis, Animal Feed Science and Technology, 2013, 183 (1-2), 1-16.
Alonso, V., Pereyra, C., Keller, L., Dalcero, A., Rosa, C., Chiacchiera, S. and Cavaglieri, L., Fungi and mycotoxins in silage: an overview, Journal of Applied Microbiology, 2013, 115, 637-643.
Cavallarin, L., Tabacco, E., Antoniazzi, S., and Borreani, G., Aflatoxin accumulation in whole crop maize silage as a result of aerobic exposure. Journal of the Science of Food and Agriculture, 2011, 91, 2419-2425.
Shreve, B., Thiex, N., and Wolf, M., National Forage Testing Association Reference Method: Dry Matter by Oven Drying for 3 Hours at 105°C, NFTA Reference Methods, National Forage Testing Association, Omaha, NB, USA, 2006.
Jajić, I., Bursić, V., Jakšić, S., Vuković, G., and Krstović S., Aflatoxin presence in Serbian poultry feed during 2013-2014 period, Contemporary Agriculture, 2015, 64 (1-2), 95-99.
Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs, Official Journal of the European Union, 2006, L70, 12-34.
Republic Hydrometeorological Service of Serbia, Agrometeorološki uslovi u proizvodnoj 2016/2017 godini na teritoriji Republike Srbije, 2017. Home page address: http://www.hidmet.gov.rs
Bočarov-Stančić, A., Izveštaj o mikrobiološkim i toksikološkim pretragama silaze, A. D. Bio-ekološki centar, Zrenjanin, 2005.
Lević, J., Ðuragić, O., Kos, J., Varga, J., and Bagi F., The occurrence of aflatoxins in Serbia - from feed to food, The second North and East European Congress on Food, Kiev, Ukraine, May 26–29, 2013, pp. 77.
Lang, C., Sipos, W., Schuh, M., and Schmoll, F., Analysis of the Fusarium toxins: Deoxynivalenol and zearalenone in Austrian feeds of the crop years 2002 and 2003, Mycotoxin Research, 2005, 21 (1), 29-31.
Van Pamel, E., Berbeken, A., Vlaemynck, G., De Boever, J., and Daeseleire, E., Ultrahigh-performance liquid chromatographic-Tandem mass spectrometric multimycotoxin method for quantitating 26 mycotoxins in maize silage, Journal of Agricultural and Food Chemistry, 2011, 59, 9747-9755.
Rasmussen, R. R., Mycotoxins in maize silage: Detection of toxins and toxicological aspects, PhD thesis Technical University of Denmark, 2010, pp. 116.
Lanier, C., Richard, E., Heutte, N., Picquet, R., Bouchart, V., and Garon, D., Airborne molds and mycotoxins associated with handling of corn silage and oilseed cakes in agricultural environment, Atmospheric Environment, 2010, 44 (16), 1980-1986.
Eckard, S., Wettstein, F. E., Forrer, H. R., and Vogelgsang, S., Incidence of Fusarium Species and Mycotoxins in Silage Maize, Toxins, 2011, 3 (8), 949-967.
Gonzalez Pereyra, M. L., Alonso, V. A., Sager, R., Morlaco, M. B., Magnoli, C. E., Astoreca, A. L., Rosa, C. A. R., Chiacchiera, S. M., Dalcero, A. M., and Cavaglieri, L. R., Fungi and selected mycotoxins from pre- and postfermented corn silage, Journal of Applied Microbiology, 2007, 104, 1034-1041.
Signorini, M. L., Gaggiotti, M., Molineri, A., Chiericatti, C. A., Zapata de Basílico, M. L., Basílico, J. C., and Pisani, M., Exposure assessment of mycotoxins in cow's milk in Argentina. Food and Chemical Toxicology, 2012, 50 (2), 250-257.
El-Shanawany, A. A., Mostafa, M. E., Barakat, A., Fungal populations and mycotoxins in silage in Assiut and Sohag governorates in Egypt, with a special reference to characteristic Aspergilli toxins. Mycopathologia, 2005, 159, 281-289.