Dmrt1 and Cyp17a1 Protein Detection and Relative Quantification in Best Beluga Hybrid Sturgeon Gonads
Keywords:
gonad development, Western Blot, sturgeons, aquacultureAbstract
Sturgeons are highly valuable species from both a conservation and economic point of view. Their very sought-after caviar extracted from mature females has both a rich culture around it and opens the way for interesting research. Because of the advanced age at which females and males could be identified in aquaculture it is important to develop a set of molecular markers that could make the distinction easy. For this, a better understanding of the sexual development of sturgeons is necessary. We selected the Dmrt1 (doublesex and mab-3 related transcription factor 1) and the Cyp17a1 (17α-hydroxylase) markers with the scope of investigating the gonad development of sturgeon individuals. Both markers appear to be expressed in the gonad but no statistically significant difference in expression is observed between females and males. This could mean that the markers are equally involved in female and male sexual development at the stage of sampling. This study paves the way for a better understanding of sturgeon sexual development.
References
Gessner, J., Freyhof, J., Kottelat, M., Acipenser gueldenstaedtii, Acipenser stellatus, Acipenser ruthenus, Huso huso. The IUCN Red List of Threatened Species 2010: e.T232A13042340. http://dx.doi.org/10.2305/IUCN.UK.20101.RLTS.T232A13042340.en. Downloaded on 29 January 2018.
Moniot, B., Berta, P., Scherer, G., Südbeck, P., Poulat, F., Male specific expression suggests role of DMRT1 in human sex determination, Mechanisms of Development, 2000, 91, 323–325.
Smith, C.A., Roeszler, K.N., Ohnesorg, T., Cummins, D.M., Farlie, P.G., Doran, T.J., Sinclair, A. H., The avian Z-linked gene DMRT1 is required for male sex determination in the chicken, Nature, 2009, 461, 267–271.
Kettlewell, J.R., Raymond, C.S., Zarkower, D., Temperature-dependent expression of turtle DMRT1 prior to sexual differentiation, Genesis, 2000, 26, 174–178.
Shibata, K., Takase, M., Nakamura, M., The DMRT1 expression in sex-reversed gonads of amphibians, General and Comparative Endocrinology, 2002, 127, 232–241.
Marchand, O., Govoroun, M., D’Cotta, H., McMeel, O., Lareyre, J.J., Bernot, A., Laudet, V., Guiguen, Y., DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss, Biochimica et Biophysica Acta, 2000, 1493, 180–187.
Leng, X.Q., Du, H.J., Li, C.J., Cao, H., Molecular characterization and expression pattern of dmrt1 in the immature Chinese sturgeon Acipenser sinensis, Journal of Fish Biology, 2016, 88, 567-579.
Hinfray, N., Nobrega, R.H., Caulier, M., Baudiffier, D., Maillot-Marechal, E., Chadili, E., Palluel, O., Porcher, J.M., Schulz, R., Brion, F., Cyp17a1 and Cyp19a1 in the zebrafish testis are differentially affected by oestradiol, Journal of Endocrinology, 2013, 216, 375-388.
Dalla Valle, L., Ramina, A., Vianello, S., Belvedere, P., Colombo, L., Cloning of two mRNA variants of brain aromatase cytochrome P450 in rainbow trout (Oncorhynchus mykiss Walbaum), Journal of Steroid Biochemistry and Molecular Biology, 2002, 82, 19–32.
Wang, Y., Ge, W., Cloning of zebrafish ovarian P450c17 (CYP17, 17α-hydroxylase/17, 20-lyase) and characterization of its expression in gonadal and extra-gonadal tissues, General and Comparative Endocrinology, 2004, 135, 241-249.
Yarmohammadi, M., Pourkazemi, M., Kazemi, R., Yazdani Sadati, M.A., Hallajian, A., Saber, M.H., Sex steroid level and sexual dimorphism expression of genes in gonads of the great sturgeon Huso huso Linneaus, 1758 during maturity developmental stages, Aquaculture Research, 2017, 48, 1413-1429.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent, Journal of Biological Chemistry, 1951, 193, 265-275.
Amberg, J.J., Goforth, R., Stefanavage, T., Sepúlveda, M.S., Sexually dimorphic gene expression in the gonad and liver of shovelnose sturgeon (Scaphirhynchus platorynchus), Fish Physiology and Biochemistry, 2010, 36, 923-932.
Wang, W., Zhu, H., Dong, Y., Tian, Z., Dong, T., Hu, H., Niu, C., Dimorphic expression of sex-related genes in different gonadal development stages of sterlet, Acipenser ruthenus, a primitive fish species, Fish Physiology and Biochemistry, 2017, 43, 1557-1569.
Berbejillo, J., Martinez-Bengochea, A., Bedó, G., Brunet, F., Volff, JN., Vizziano-Cantonnet, D., Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii, Molecular Reproduction and Development, 2012, 79, 504−516.
