RP1S7 – A Possible Marker for Sturgeon Hybrids Identification
Keywords:
sturgeons, hybridization, nuclear markers, RP1S7Abstract
The Acipenseridae family includes sturgeon species that have the ability to easily generate interspecies hybrids in both natural and aquaculture conditions. For the differentiation between hybrids and pure species, mitochondrial markers have been used, but they have not proven effective. Nuclear markers, such as the RP1S7 marker, encoding the S7 ribosomal protein, show to be more effective in achieving this differentiation. The purpose of this study was to evaluate the capacity of this nuclear marker in discriminating between pure sturgeon species and their interspecies hybrids. DNA samples were isolated from fin fragments. The RP1S7 marker was amplified by PCR and the obtained amplicons were analysed by agarose gel electrophoresis and sequencing. In order to determine the accuracy of the primers and to validate this marker, the sequences were compared with similar ones from GenBank. The resulting electrophoretic profiles showed differences that can be used to distinguish between pure sturgeon species and their interspecies hybrids. Therefore, the RP1S7 marker can be included in a panel of DNA markers for molecular identification of sturgeon species and their hybrids.
References
Bemis, W. E., and Kynard, B., Sturgeons rivers: an introduction to acipenseriform biogeography and life history, Environmental Biology of Fish, 1997, 48, 167-183.
Costache, M., Dudu, A., and Georgescu, S.E., Low Danube sturgeon identification using DNA markers. In: Analysis of Genetic Variation in Animals. M. Caliskan, InTech, 2012, pp. 243-268.
Birstein, V.J., Hanner, R., and DeSalle, R., Phylogeny of the Acipenseriformes: cytogenetic and molecular approaches. Environmental Biology of Fish, 1997, 48, 127-155.
Dudu, A., Macarie, R.D., Burcea, A., Georgescu, S.E., and Costache, M., Identification of bester hybrid and its parental species (♀ Huso huso Linnaeus, 1758 and ♂ Acipenser ruthenus Linnaeus, 1758) by nuclear markers, Scientific Papers: Animal Science and Biotechnologies, 2015, 48(1), 81-85.
Rehbein, H., Difference of fish species by PCR-based DNA analysis of nuclear genes, European Food Research and Technology, 2013, 236, 979-990.
Chow, S., and Hazama, K., Universal PCR primers for S7 ribosomal protein gene introns in fish, Molecular Ecology, 1998, 7, 1255-1256.
Annilo, T., Jelina, J., Pata, I., and Metspalu, A, Isolation and characterization of the mouse ribosomal protein S7 gene, Biochemistry and Molecular Biology International, 1998, 46, 287-295.
Carvalho-Costa, L.F., Piorski, N.M., Willis, S.C., Galetti, Jr. P.M., and Ortí, G., Molecular systematics of the neotropical shovelnose catfish genus Pseudoplatystoma Bleeker 1862 based on nuclear and mtDNA markers, Molecular Phylogenetics and Evolution, 2011, 59, 177-194.
Keck, B.P., and Near, T.J., Assessing phylogenetic resolution among mitochondrial, nuclear, and morphological datasets in Nothonotus darters (Teleostei: Percidae), Molecular Phylogenetics and Evolution, 2008, 46, 708–720.
Liu, D., Guo, H.-I., Tang, W.-Q., and Yang, J.-Q., Comparative Evolution of S7 Intron 1 and Ribosomal Internal Transcribed Spacer in Coilia nasus (Clupeiformes: Engraulidae), International Journal of
Molecular Sciences, 2012, 13, 3085-3100.
Taggart, J., Hynes, R., Prodohl, P., and Ferguson, A., A simplified protocol for routine total DNA isolation from salmonid fishes, Journal of Fish Biology, 1991, 40(6), 963-965.
Hall, T.A., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT, Nucleic Acids Symposium Series, 1999, 41, 95-98.
Clark, K., Karsch-Mizrachi, I., Lipman, D.J., Ostell, J., Sayers, E.W., GenBank, Nucleic Acids Research, 2016, 44, 67-72.
