CHANGES INDUCED BY SOME NUTRITIONAL FACTORS ON TIBIAL BONE STRUCTURE IN PIG

Abstract

The problems approached by our team are represented by the involvement of
different sources and levels of calcium, namely calcium carbonate, fructoborate and
alfalfa, in the mineralization of bone tissue. So, we made fix histological
preparations of tissue fragments taken from tibia bone, from 9 pigs belonging to
three batches: the control batch, where calcium was provided in a proportion of 1%
through calcium carbonate, experimental batch 1, where calcium was provided in a
proportion of 1.04% through fructoborate, on a calcium carbonate support, and
experimental batch 2, where calcium was provided in a proportion of 1.13% through
fructoborate + alfalfa, on a calcium carbonate support. The histomorphometric
parameters assessed were represented by the volume of bone trabeculae (BV/TV, %)
or the percentage of bone tissue in a given volume and the mean width of bone
trabeculae. At the same time, in order to establish fructoborate and alfalfa
implication in bone mineralization, we supervised the presence and activity of
osteoblasts respectively osteoclasts. In the case of the experimental batch 1, the
histomorphometric study shows an increase of bone trabeculae dimension, with a
mean width of about 142,5μ µ, and also an increase of their mean volume, which is
about 37,11%. The trabecular system is dense and present mineralised and ossified
territory where are formed by osteoclasts with osteocytes. Peritrabeculary are
presented active osteoblasts which are involved in plurilamellar stratification by
deposition of young collagen. In the case of individuals from experimental batch 2,
trabeculae mean width is about 140,7μ, while their volume is 35,62%.