POLYMORPHISM OF STEAROYL-COENZYME A DESATURASE GENE IN SLOVAK PINZGAU CATTLE
Keywords:
Pinzgau cattle, PCR-RFLP, PCR-SSCP, SCD geneAbstract
The work was oriented to identification of SCD gene polymorphism in
population of Slovak pinzgau cattle. The material involved 91 cows of
pinzgau breed kept in Slovakia. Bovine genomic DNA was isolated by fenolchlorophorm deproteinization and ethanol precipitation. The polymorphism
of SCD gene was detected by PCR-SSCP method. For the checking of
correct genotyping was used PCR-RFLP method. The PCR products were
digested with SatI restriction enzyme. The mutant allele C (Ala) was detected
by 205bp, 70 bp and 40 bp fragments and the wild allele T (Val)was detected
by 205 bp and 117 bp fragments on 3 % agarose gel.
In the total population included in the study was detected homozygous
genotype CC ( 0.31 ), heterozygous genotype CT (0.42) and homozygous
genotype TT( 0.27 ). The frequency of occurrence both alleles proved gently
of predominance of mutant allele C – 0.52 before wild allele T – 0.48 .
References
Bassam, B. J., Anollés, G. C., Gresshoff, P. M., 1991, Fast and sensitive
silver staining of DNA in polyacrylamide gels, Anal. Biochem., 196, 80–83
Campbell, E. M. G., Gallagher, D. S., Davis, S. K., Taylor, J. F., Smith, S.
B., 2001, Mapping of the bovine stearoyl coenzyme A desaturase (SCD)
gene to BTA26, J. Anim. Sci., 79, 1954-1955
Enoch, H. G., Catala, A., Strittmatter, P., 1976, Mechanism of rat
microsomal stearoyl-CoA desaturase, J. Biol. Chem., 251, 5095–5103
Frank, M. Y., Naxin, H., Yong, Q. G., Ming-cheng, L., Yaqin, M., Dave,
H., Gerard, R L., Dvorak, J., Anderson, D. O., 2008, BatchPrimer3: a high
throughput web application for PCR and sequencing primer designi, BMC
Bioinformatics, 253
Green, P., Falls, K., Crooks, S., 1990, Documentation for CRIMAP.
version 2.4. Washington University School of Medicine, St. Louis.
Islas-Trejo, A., Johnson., A F., Medrano, J. F., 2002, Genomic structure
and expression of the bovine stearoyl-CoA desaturase gene, Proc. Plant,
Animal and Microbe Genomes Conference, San Diego, CA, 766.
Kim, Y. C., Ntambi, J. M., 1999, Regulation of stearoyl-CoA desaturase
genes: role in cellular metabolism and preadipocyte differentiation,
Biochem Biophys Res Commun, 266, 1–4
Mele, M., Conte, G., Castiglioni, B., Chessa, S., Macciotta, N. P. P., Serra,
A., Buccioni, A., Pagnacco, G., Secchiari, P., 2007, Stearoyl-Coenzyme A
Desaturase Gene Polymorphism and Milk Fatty Acid Composition in
Italian Holsteins, J. Dairy Sci., 90, 4458–4465
Meluš, V., Kasarda, R., Kadlečík, O., Trakovická, A., 2007,
Polymorfizmus génu SCD pinzgauského dobytka, Acta fytotechnica et
zootechnica, 10, 67 - 69
Moioli, B., Contarini, G., Avalli, A., Catillo, G., De Matteis, G., Masoero,
G., Napolitano, F., 2007, Short Communication: Effect of StearoylCoenzyme A Desaturase Polymorphism on Fatty Acid Composition of
Milk, J. Dairy Sci., 90, 3553–3558
Palmquist, D. L., Beaulieu, A. D., Barbano, D. M., 1993, Feed and animal
factor influencing milk fat composition., J. Dairy Sci., 76, 1753–1771
Smith, S. B., Yang, A., Larsen, T. W., Tume, R. K., 1998, Positional
analysis of triacylglycerols from bovine adipose tissue lipids varying in
degree of unsaturation, Lipids, 33, 197–207
Taniguchi, M., Utsugi, T., Oyama, K., Mannen, H., Kobayashi, M.,
Tanabe, Y., Ogino, A., Tsuji, S., 2004, Genotype of stearoyl-CoA
desaturase is associated with fatty acid composition in Japanese Black
cattle, Mamm. Genome, 14, 142–148
Vincze, T., Posfai, J., Roberts, R.J., 2003, NEBcutter: a program to cleave
DNA with restriction enzymes, Nucleic Acids Res., 31, 3688-3691
Yang, A., Larsen, T. W., Smith, S. B., Tume, R. K., 1999, Desaturase
activity in bovine subcutaneous adipose tissue of different fatty acid
composition, Lipids, 34, 971–978
