ANTIBIOTIC RESISTANCE OF FAECAL ENTEROCOCCI IN DOMESTIC POULTRY AND BROILERS FROM COMMERCIAL FARMS
Keywords:
Enterococcus sp., antibiotic resistance, broilers, hensAbstract
The aim of this study was to determine the prevalence and antibiotic resistance
of enterococci isolated from domestic poultry, laying hens from ecological farm and
broilers from commercial farms. Susceptibilities of isolated enterococci were tested
using the disk diffusion method. All isolates showed some degree of multiple
antibiotic resistances. The resistance to compound sulphonamides (91.891%),
tetracycline (51.351%) and streptomycin (37.837%) was the most frequent. Of all
isolates of Enterococcus strains 83.783% were susceptible to enrofloxacin. The
susceptibility of isolates from broilers and laying hens from ecology farm to
enrofloxacin were significantly higher (P<0.05) than in isolates from domestic
poultry. Between the others data were not significant differences.
References
Apajalahti, J., Kettunen, A., Graham, H., 2004, Characteristics of the
gastrointestinal microbial communities, with special reference to chicken, World’s
Poultry Science Journal, 60, 223–232
CLSI, 2004, CLSI/NCCLS M44S1 Zone Diameter Interpretive Standards
and Corresponding Minimal Inhibitory Concentration (MIC) Interpretive
Breakpoints; Informational Supplement, M44-S1, First Edition, Clinical and
Laboratory Standards Institute, Wayne, USA, 2006
da Costa, P. M., Oliveira, M., Bica,, A., Vaz-Pires, P., Bernardo, F., 2007,
Antimicrobial resistance in Enterococcus spp. and Escherichia coli isolated from
poultry feed and feed ingredients, Veterinary Microbiology, 120, 122–131
Franz, C. M. A. P., Holzapfel, W. H., Stiles, M., 1999. Enterococci at the
crossroads of food safety?, Internet Journal of Food Microbiology, 47,1–24
Huys, G., D’Haene, K, Collard, J. M., Swings, J., 2004, Prevalence and
Molecular Characterization of Tetracycline Resistance in Enterococcus Isolates
from Food, Applied and Environmental Microbiology, 70, 1555–1562
Khachatryan, A. R., Besser,T. E., Hancock, D. D., Call, D. R., 2006, Use
of a nonmedicated dietary supplement correlates with increased prevalence of
streptomycin-sulfa-tetracycline-resistant Escherichia coli on a dairy farm, Applied
Environmental Microbiology, 72, 4583–4588.
Klare, I., Konstabel, C., Badstubner, D., Werner, G., Witte. W., 2003,
Occurrence and spread of antibiotic resistances in Enterococcus faecium, Internet
Journal of Food Microbiology, 88, 269–290
Klein, G., 2003, Taxonomy, ecology and antibiotic resistance of
enterococci from food and the gastro-intestinal tract, Internet Journal of Food
Microbiology, 88,123–131.
Kolář, M., Bartoň, J., Vágnerová, I., Hájek, V., Bzdil, J., Kohnová, H.,
Typovská, H. 2000, Occurence of vancomycin-resistant enterococci in hens in the
central region of Morava, Veterinary Medicine-Czech, 45, 93-97
Murray, B. E., 1990, The life and times of the Enterococcus, Clinical
Microbiology Reviews, 3, 46-65
Nandi, S., Maurer, J. J., Hofacre, C., Summers, A. O., 2004. Grampositive
bacteria are a major reservoir of class 1 antibiotic resistance integrons in poultry
litter. The Proceedings of the National Academy of Sciences, 101, 7118–7122.
