The Rate of Tetraploidy Determination in Rainbow Trout (Oncorhynchus Mykiss) in Embryonated Eggs

Authors

  • Ioan Bencsik Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania
  • Nicolae Pacala Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania
  • Gabi Dumitrescu Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania
  • Dorel Dronca Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania
  • Jana Stanculet SC Detergenti SA, 300571 Timisoara,,Calea Stan Vidrighin,No. 5, Romania
  • Liliana Petculescu-Ciochina Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania
  • Liliana Boca Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645 Timisoara,, Calea Aradului Street, No. 119, Romania

Keywords:

heat shock, rainbow trout, tetrapoidy, kariotype analysis

Abstract

Polyploidy at fish is characterized by modification of normal diploid chromosome set (2n) to triploid (3n), tetraploid (4n) etc. Experiments were carried out on biological material from rainbow trout (Oncorhynchus mikiss) during the natural period of reproduction. Polyploidy can be induced by exposing the eggs to heat shock. The highest levels of embryo losses are observed in the first two decades of incubation. The success rate of the tetrapolidy induction after 6 hours after fertilization evaluated by karyotype analysis was 66.67%. During the whole incubation period for the experimental group the losses were evaluated at 39.86% from the incubated eggs, and for the control group at 13.48%.

References

Kapuscinski A., R., Miller L., M., Genetic guidelines for fisheries management, University of Minesota, 2007,p.66-68.

Beaumont, A., Hoare, K., Biotehnology and genetics in fisheries and aquaculture, Blakwell Publishing, 2003.

Bohl, M., Zucht und Production von Suβwasserfischen, Verglas Union Agrar,1999, p. 555-558.

Colihueque, N., Iturra, P., Diaz, N., Veloso, A., Estay, F., Karyological analysis and identification of heterochromosomes in experimental gynogenetic offspring of rainbow trout (Oncorhynchus mykiss, Walbaum) Rev.Brasil.Genet. 1992,15,3,535-546.

Feist, G., Scheck, C. B., Chrrett, A., Controlling the sex of salmonids, Oregon Sea Grant, 1996,ISBN 1-881826-10-4.

Phillips, R.B., Zajicek, K.D., Ihssen, P.E. and Johnson, O. Application of silver staining to the identification of triploid fish cells., Aquaculture 1986, 54, 313–319

Chourraut, D., Tetraploidy induced by heat shoks in the rainbow trout (Salmo gairdneri R.), Reprod.Nutr. Develop., 1982,22,(3) 569-574.

Benfey, T.J., Sutterlin, A.M. and Thompson, R.J. Use of erythrocyte measurements to identify triploid salmonids. Canadian Journal of Fisheries and Aquatic Sciences, 1984,41, 980–984

Garcia-Abiado, M.A.R., Dabrowski, K., Christensen, J.E., Czesny, S. and Bajer, P., Use of erythrocyte measurements to identify triploid saugeyes. North American Journal of Aquaculture, 1999,61, 319–325

Quillet, E., Aubard, G., Queau, I., Mutattion in a sex-determining gene in rainbow trout Detection and genetic analysis, The American Genetic Association, 2002,93:91–99

Dunham, R.A., Aquaculture and fisheries biotehnologie, Genetic approaches, CABI Publishing, Cambridge, USA,2004,. ISBN 0 85199-596-9

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Published

2023-11-01