Effect of Probiotics and Humic Acid on Egg Production and Quality Parameters of Laying Hens Eggs
Keywords:
egg quality, egg production, humic acid, laying hens, probioticsAbstract
The aim of our experiment was to evaluate the influence of probiotic preparation based on lactobacillus; probiotic preparation based on enterococci or humic acid on egg production and selected internal egg quality parameters of laying hens’ hybrid Lohman Brown Lite. For housing hens (n=60) three storey enriched battery cage was used in which hens were divided in groups (n=15). In the control group of hens complete feed mixtures without any additions were fed. In the first experimental group complete feed mixture was enriched with probiotic enterococci based preparation in a dose of 0.5 g x kg-1. In the second experimental group probiotic lactobacilli based preparation was added to the feed mixture at a dose of 0.5 g x kg-1. The third experimental group was enriched with 0.5% concentration of humic acid. All groups were fed ad libitum. Egg production and egg weight were recorded daily. Complete analysis of the table egg quality was used to evaluate quality parameters: yolk weight (g), yolk index, yolk colour (HLR), albumen weight (g), Haugh Units (HU), and albumen index). The results show that supplementation of feed mixture with both kind probiotics as well as humic acid increased egg production (p>0.05) (values of average intensity of laying in the order of the groups: 90.3; 92.0; 91.6 and 92.4%). The addition of probiotics or humic acid only slightly influenced egg weight (p>0.05). The addition of both kind of probiotics influenced non-significant positive albumen index (p>0.05). The supplement of probiotics on lactobacilli based statistically significantly increased Haugh Units (p£0.05). The supplement of humic acid statistically significantly increased albumen index and Haugh Units (p£0.05). The others qualitative parameters of egg internal content were with probiotics or humic acids addition insignificantly influenced (p>0.05). Doses of supplements used in this study did not significant negatively influenced monitored egg quality parameters. Based on these findings and the beneficial effects of substances on the poultry health confirmed by other authors we recommend use of these substances as supplements to the feed mixtures for laying hens.
References
Applegate, T. J., Klose, V., Steiner, T., Ganner, A., Schatzmayr, G., Probiotics and phytogenics for poultry: myth or reality?, The Journal of Applied Poultry Research, 2010, 19, 194-210.
Arpášová, H., Fytobiotiká ako náhrada antibiotických stimulátorov rastu aich vplyv na úžitkovosť akvalitu vajec sliepok znáškového typu. Nitra, SPU, 2011, 101 s. ISBN 978-80-552-0555-7.
Musa, H. H., Wu, S. L., Zhu, C. H., Seri, H. I., Zhu, G. Q., The potential benefits of probiotics in animal production and health, Journal of Animal and Veterinary Advances, 2009, 8(2), 313-321.
Kačániová, M., Nováková, I, Haščík, P. et al., Aplikácia probiotík a ich vplyv na ekosystém kurčiat, Bezpečnosť akvalita surovín apotravín, Nitra: SPU, 2009, 38.
Perumalla, A. V. S., Hettiarachchy, N. S., Ricke, S. C., Current Perspectives on Probiotics in Poultry Preharvest Food Safety, Direkt-fed Microbials and Prebiotics for Animals, 2012, 89-120.
Shini, S., Shini, A., Blackall, P. J., The potential for probiotics to prevent reproductive tract lesions in free-range laying hens, Animal Production Science, 2013, 53(12), 1298-1308.
Rath, N.C., Richards, M. P., Huff, W. E., Huff, G. R., Balog, J. M., Changes in the tibial growth plates of chickens wiyh thiraminduced dyschondroplasia, J. Comp. Pathol., 2005, 133, 41-52.
Vaškova, J., Veliká, B., Pilátová, M., Kron, I., Vaško, L., Effect of humic acid in vitro, Vitro Cell. Dev. Biol.-Animal., 2011, 47, 376-382.
Ozturk, E., Coskun, I., Ocak, N. et al., Performance, meat quality, meat mineral contents and caecal microbial population responses to humic substances administered in drinking water in broilers, British Poultry Science, 2014, 55(5), 668-674.
Nagaraju, R., Reddy, B. S., Gloridoss, R., Suresh, B. N., Ramesh, C., Effect of dietary supplementation of humic acids on performance of broilers, Indian Journal of Animal Sciences, 2014, 84(4), 447–452.
Černeková, D., Humínové kyseliny, Chovateľ, 2009, 45(3), 10-11.
Zraly, Z., Pisarikova, B., Navratilova, M., The effect of humicacid on mercury accumulation in chicken organs and muscle tissues, Czech Journal of Animal Scienc., 2008, 53(11), 472-478.
Bubel, F., Dobrzanski, Z., Gawel, A., Effect of humic-plant feed preparations on biochemical blood parameters of laying hens in deep litter housing systém, Polish Journal of Veterinary Sciences, 2015, 18(1), 131-139.
Gladkowski, W., Kielbowicz, G., Chojnacka, A., Gil, M., Trziszka, T., Dobrzanski, Z., Wawrzenczyk, C., Fatty acid composition of egg yolk phospholipid fractions following feed supplementation of Lohmann Brown hens with humic-fat prepatations, Food Chem., 2011, 126, 1013-1018.
Zhang, J. L., Xie, Q. M., Ji, J., Yang, W. H., Wu, Y. B., Li, C., Ma, J. Y., Bi, Y. Z., Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens, Poultry Science, 2012, 91(11), 2755-2760
Zhang, Z. F., Kim I. H., Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens, J Anim Sci., 2013, 91(10), 4781-7.
Capcarova, M., Chmelnicna, L., Kolesarova, A., MassanyI, P., Kovacik, J., Effects of Enterococcus faecium M 74 strain on selected blood and production parameters of laying hens, British Poultry Science, 2010, 51(5), 614-620.
Xu, C. L., Ji, C., Ma, Q., Hao, K., Jin, Z.Y., Li, K., Effects of a dried Bacillus subtilis culture on egg quality, Poultry Science, 2006, 85(2), 364-368.
Gallazzi, D., Giardini, A., Mangiagalli, M. G., Marelli, S.,Ferrazzi, V., Orsi, C., Cavalchini, L. G., Effects of Lactobacillus addophilus D2/CSL on laying hen performance, Italian Journal of Animal Science, 2008, 7(1), 27-37.
Mo, E. K., Lee, J. H., Xu, B. J., Lee, B. D., Moon, Y. J., Sung, C. K., Effect of Pichia farinosa SKM-1, Pichia anomala SKM-T, and Galactomyces geotrichum SJM-59 on ammonia reduction and laying performance, Journal of Microbiology and Biotechnology, 2004, 14(1), 22-28.
Balevi, T., Ucan, U. S., Coskun, B., Kurtoglu, V., Cetingul, I. S., Effect of dietary probiotic on performance and humoral immune response in layer hens, British Poultry Science, 2001, 42(4), 456–461.
Rosa, A., Sanmiguel, P., Barragán, R., Supplementation with humic substances in laying hens during molt, CES Medicina Veterinaria y Zootecnia, 2012, 9(2), 169-178.
Bong, Seok ho., Production of Multiple Probiotics and the Performance of Laying Hens by Proper Level of Dietary Supplementation, Korean Journal of Poultry Science, 2011, 38(3), 173-179.
Sobczak, A., Kozlowski, K., the Effect Of A Probiotic Preparation Containing Bacillus Subtilis Atcc Pta-6737 On Egg Production And Physiological Parameters Of Laying Hens, Annals of Animal Science, 2015, 15,(3), 711-723.
Lei, K., Li, Y. L., Yu, D. Y., Rajput, I. R., Li, W. F. Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzyme activities, and intestinal barrier function of laying hens, Poultry Science, 2013, 92(9), 2389-2395.
Kang, H. K., Kim, J. H.,Kim, C. H., Effect of dietary supplementation of Lactobacillus-fermented and non-fermented sea buckthorn on the laying performance and meat lipid peroxidation of Hy-line Brown laying hens, European Poultry Science, 2015, 79.
Ribeiro, V., Jr., Albino, L. F. T., Rostagno, H. S., Barreto, S. L. T., Hannas, M. I., Harrington, D., De Araujo, F. A., Ferreira, H. C., Jr., Ferreira, M. A., Effects of the dietary supplementation of Bacillus subtilis levels on performance, egg quality and excreta moisture of layers. In Animal Feed Science and Technology, 2014, 195, 142-146.
Ramasamy, K., Abdullah, N., Jalaludin, S., Wong, M.,Ho, Y. W., Effects of Lactobacillus cultures on performance of laying hens, and total cholesterol, lipid and fatty acid composition of egg yolk, Journal of the Science of Food and Agriculture, 2009, 89(3), 482-486.
Hakan, K. B., Gultekin, Y., Sizmaz, O., Parameters of Laying Hens, Brazilian Journal of Poultry Science, 2012, 14(4), 283-289.
Yörük, M. A., Gül, M., Hayirli, A., Macit, M., The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens, Poultry Science, 2004,. 83(1), 84-88.
Loh, T. Ch., Choe, D. W., Foo, H. L.,. Effects of feeding different postbiotic metabolite combinations produced by Lactobacillus plantarum strains on egg quality and production performance, faecal parameters and plasma cholesterol in laying hens, Bmc Veterinary Research, 2014, 10.
Abdelqader, A., Irshaid, R., Al-Fataftah, A., Effects of dietary probiotic inclusion on performance, eggshell quality, cecal microflora composition, and tibia traits of laying hens in the late phase of production, Tropical Animal Health and Production, 2013, 45(4), 1017-1024.
Tang, S. G. H., Sieo, Ch., Kalavathy, R., Chemical Compositions of Egg Yolks and Egg Quality of Laying Hens Fed Prebiotic, Probiotic, and Synbiotic Diets, Journal of Food Science, 2015, 80(8), p. C1686-C1695.
Yalcin, S., Ergun, A., Ozsoy, B.,Yalcin, S., Erol, H., Onbasilar, I., The effects of dietary supplementation of L-carnitine and humic substances on performance, egg traits and blood parameters in laying hens, Asian-Australasian Journal of Animal Sciences, 2006, 19(10), 1478-1483.
Panda, A. K., Reddy, M. R., Rao, S. V. R., Praharaj, N. K., Production performance, serum/yolk cholesterol and immune competence of White Leghorn layers as influenced by dietary supplementation with probiotic, Tropical Animal Health and Production, 2003, 35(1), 85–94.
Chumpawadee, S., Chantiratikul, A., Sataweesuk, S., Effect of Dietary Inclusion of Cassava Yeast as Probiotic Source on Egg Production and Egg Quality of Laying Hens, International Journal of Poultry Science, 2009, 8(2), 195-199.
Yousefi, M., Karkoodi, K., Effect of probiotic Thepax and Saccharomyces cerevisce Supplementation on Performance and Egg Qaulity of Laying hen, International Journal of Poultry Science, 2007, 6(1), 52-54.
Ergin, O., Isa, C. ,Nuh, O., Guray, E., Effects of dietary humic substances on egg production and egg shell quality of hens after peak laying period, African Journal of Biotechnology, 2009, 8(9), 1155-1159.
Hong, J. W., Kim, I. V. ,Kwon, O. S., Han, Y. K.,Lee, S. H., Influence of probiotics supplementation on egg quality and excretal noxious gas in laying hens, Journal of Animal Science and Technology, 2002, 44, (2), 213–220.
Yalcin, S., Guclu, B. K., Oguz, F. K., The usage of enzyme, probiotic and antibiotic in laying hen rations, Ankara Universitesi Veteriner Fakultesi Dergisi. 2002, 49(2), 135–141.
Kalavathy, R., Abdullah, N., Jalaludin, S., Wong, C. M. V. L., Ho, Y. W., Effects of Lactobacillus cultures on performance and egg quality during the early laying period of hens, Journal of Animal and Feed Sciences, 2005, 14(3), 537-547.
Yodseranne, R., Chantsavang, S., Bunchasuk, C. Effect of dietary supplementation of mixed culture compost, plant and animal bioextract compost on layer´s productive performance and egg quality, Animal and Veterinary Medicine, 2003, 119–126.