Determination of Spoilage Yeasts in Different Red and White Wines
Keywords:
yeasts, wine samples, MALDI-TOF MSAbstract
The aim of this study was to isolate and identify yeast species in wine samples from five different countries, and
determine the genera of spoilage yeasts. One hundred and thirty four (no. 134) wine samples (86 white and 48 red
wine); (30 bottled, 104 tank wine) from five countries Slovakia (no. 53), Czech Republic (no. 17), Hungary (no. 6),
Spain (no. 32) and Italy (no. 26) were used in this study. Yeasts were cultivated on Malt extract agar supplemented
with bromocresol green. After cultivation, yeasts were identified on species level by MALDI-TOF mass
spectrometry. We identify 10 yeasts genera from total of 285 isolates; Saccharomyces (97 isolates, 1 species), Pichia
(120 isolates, 5 species), Kregervanrija (6 isolates, 1 species), Zygosaccharomyces (32 isolates, 2 species), Candida
(13 isolates, 2 species), Rhodotorula (4 isolates, 1 species), Wickerhamomyces (7 isolates, 1 species) Meyerozyma (4
isolates, 1 species) and genera with only 1 isolate and species: Torulaspora, and Cyberlindnera.
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